Expression of soluble human tumor necrosis factor receptor Ⅰ in Aspergillus niger  

作　　者：LI Min, HONG Bin & LI YuanInstitute of Medicinal Sciences & Peking Union Medical College, Beijing 100050, China 

出　　处：《Chinese Science Bulletin》2001年第11期918-921,共4页

基　　金：the National High Biotech Youth Fund Program.

摘　　要：The cDNA of soluble human tumor necrosis factor receptor I (sTNFRI) was inserted into fusion-protein expression plasmid pIGF of A. niger to construct fusion expression vector pHBC containing a KEX2 like protein processing site designed on the fusion position. Extracellular protease-deficient strain of A. niger 3.795-1-23 was transformed with pHBC. Positive clone was estimated by Southern hybridization. SDS-PAGE for protein produced by re-combinant strain showed the distinctive expression band. Western blotting indicated that the secreted protein had immunoactivity of sTNFRI.The cDNA of soluble human tumor necrosis factor receptor I (sTNFRI) was inserted into fusion-protein expression plasmid pIGF of A. niger to construct fusion expression vector pHBC containing a KEX2 like protein processing site designed on the fusion position. Extracellular protease-deficient strain of A. niger 3.795-1-23 was transformed with pHBC. Positive clone was estimated by Southern hybridization. SDS-PAGE for protein produced by re-combinant strain showed the distinctive expression band. Western blotting indicated that the secreted protein had immunoactivity of sTNFRI.

关 键 词：STNFRI ASPERGILLUS NIGER KEX2 SECRETORY EXPRESSION fusion protein. 

分 类 号：R392[医药卫生—免疫学]