EFFECTS OF TRANSIENT FOREBRAIN ISCHEMIA AND RADIX SALVIAE MILTIORRHIZAE (RSM) ON EXTRACELLULAR LEVELS OF MONOAMINE NEUROTRANSMITTERS AND METABOLITES IN THE GERBIL STRIATUM-An in vivo Microdialysis Study  

作　　者：程敬君 匡培根 吴卫平 张凤英 

机构地区：[1]Neurotransmitter Laboratory,Department of Neurology,Chinese PLA General Hospital,PLA Postgraduate Medical School,Beijing 100853,P.R.China

出　　处：《Journal of Traditional Chinese Medicine》1999年第2期135-140,共6页中医杂志（英文版）

摘　　要：The aim of this study was to investigate the effect of 30 min forebrain ischemia, followed by 120 min reperfusion on extracellular fluid (ECF) levels of dopamine (DA), norepinephrine (NE), serotonin (5-HT) and their metabolites, homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) in the striatum of gerbils, so as to obtain further information on the mechanism of Radix Salviae Miltiorrhizae (RSM)-induced neuroprotection. Microdialysis was used to sample the extracellular space. Dialysate was measured by high performance liquid chromatography with electrochemical detector (HPLC-ED). ECF DA, NE levels increased from basal levels by 282, 227 and 221 folds, by 9.14, 8.51 and 8.25 folds, respectively for the three ischemic duration (0-10; 11-20; 21-30 min). ECF DA, NE, 5-HT levels in the RSM-treated group were significantly decreased as compared with those in the control group during ischemia (PThe aim of this study was to investigate the effect of 30 min forebrain ischemia,followed by120 min reperfusion on extracellular fluid(ECF)levels of dopamine(DA),norepinephrine(NE),serotonin(5-HT)and their metabolites,homovanillic acid(HVA)and 5-hydroxyindoleacetic acid(5-HIAA)in the striatum of gerbils,so as to obtain furtherinformation on the mechanism of Radix Salviae Miltiorrhizae(RSM)-inducedneuroprotection.Microdialysis was used to sample the extracellular space.Dialysate wasmeasured by high performance liquid chromatography with electrochemical detector(HPLC-ED).ECF DA,NE levels increased from basal levels by 282,227 and 221 folds,by9.14,8.51 and 8.25 folds,respectively for the three ischemic duration(0-10;11-20;21-30min).ECF DA,NE,5-HT levels in the RSM-treated group were significantly decreased ascompared with those in the control group during ischemia(P<0.01).The results suggestedthat monoamine neurotransmitters were involved in ischemic neuron damage directly orindirectly;and that RSM plays a protective role during cerebral ischemia by attenuating thedysfunctions of monoamine neurotransmitters.

关 键 词：Animals Biogenic Monoamines Corpus Striatum Dopamine Drugs  Chinese Herbal Extracellular Space GERBILLINAE Ischemic Attack  Transient Male MICRODIALYSIS Neuroprotective Agents NOREPINEPHRINE Prosencephalon Reperfusion Injury SEROTONIN 

分 类 号：R285.5[医药卫生—中药学]