Effect of internal deletion of Myosin Ⅱ on growth and development of Dictyostelium discoideum  

作　　者：SHU Shi Taro Q. P. Uyeda LIU Aixiao LIU Guoqin and YEN LongfeiCollege of Biological Sciences, China Agricultural University, Beijing 100094, China  Rionic Design Group , National Institute for Advanced Interdisciplinary Research , Tsukuba , Ibaraki 305 - 8562 , Japan 

出　　处：《Chinese Science Bulletin》1999年第9期811-816,共6页

摘　　要：Four deletion mutant Dictyostelium myosin Ⅱ heavy chain genes, MyΔ824-941 (Δ1/3S2), MyΔ934-1454 (ΔS2), MyΔ934-1194 (ΔS2-1) and MyΔl157-1454 (ΔS2-2), were transformed by standard electroporation into mhcA- cells (T-null), a mutant Dictyostelium cell devoid of endogenous myosin Ⅱ heavy chain gene. The growth, development and formation of fruiting bodies of cells expressing those mutant myosin Ⅱ s under suspension culture were investigated by comparison with the wild type cell. The results indicate that internal deletion of myosin Ⅱ affects the growth and development of Dictyostelium. Furthermore, the longer the length of deletion, the more serious the defect in phenotype.Four deletion mutant Dictyostelium myosin Ⅱ heavy chain genes, MyΔ824-941 (Δ1/ 3S2), MyΔ934-1454 (ΔS2), MyΔ934-1194 (ΔS2-1) and MyΔ1157-1454 (ΔS2-2), were transformed by standard electroporation into mhcA- cells (T-null), a mutant Dictyostelium cell devoid of endogenous myosin Ⅱ heavy chain gene. The growth, development and formation of fruiting bodies of cells expressing those mutant myosin Ⅱ s under suspension culture were investigated by comparison with the wild type cell. The results indicate that internal deletion of myosin Ⅱ affects the growth and development of Dictyostelium. Furthermore, the longer the length of deletion , the more serious the defect in phenotype.

关 键 词：DICTYOSTELIUM MYOSIN Ⅱ DELETION MUTATION GROWTH and development. 

分 类 号：Q754[生物学—分子生物学]