Establishment of Chlamydomonas reinhardtii chloroplast expression  

作　　者：Guochang Fan Ning Su Zhonglin Zhang Xiangfu Wu Guifang Shen 

机构地区：[1]Chinese Acad Agr Sci, Biotech Res Ctr, Beijing 100081, Peoples R China [2]Beijing Inst Radiat Med, Beijing 100850, Peoples R China [3]Chinese Acad Sci, Shanghai Inst Biochem, Shanghai 200031, Peoples R China

出　　处：《Chinese Science Bulletin》1999年第18期1659-1664,共6页

摘　　要：An expression vector pACⅢ containing the chimeric gene HAV- VP3P1 and HCV-C gene has been constructed and transferred to C. reinhardtii by the biolistic method. The trans-formants have been identified by PCR, Southern-blotting, Northern-blotting and Western-blotting assays after selecting on resistant medium and incubating in the dark. The resultsAn expression vector pAC III containing the chimeric gene HAV-VP3P1 and HCV-C gene has been constructed and transferred toC. reinhardtii by the biolistic method. The transformants have been identified by PCR, Southern-blotting, Northern-blotting and Western-blotting assays after selecting on resistant medium and incubating in the dark. The results show that the chimeric gene has replaced thechlL gene ofC. reinhardtii chloroplast genome and expressed correctly under the control of theC. reinhardtii chloroplast double promoters 5′chlL-5′atpA. The analysis of SDS-PAGE indicates that the expressed protein accounts for 5.31 % of total soluble protein.

关 键 词：HAV-VP3P1 GENE HCV-C GENE C. REINHARDTII CHLOROPLAST EXPRESSION system HOMOLOGOUS recombinant. 

分 类 号：Q945[生物学—植物学]