MAP2c confers drug stability to microtubules in vivo  

作　　者：Xiaoyong Yang Junlin Teng Yang Jin Jianguo Chen 

机构地区：[1]Peking Univ, Coll Life Sci, Dept Cell Biol & Genet, Beijing 100871, Peoples R China

出　　处：《Chinese Science Bulletin》1999年第23期2162-2168,共7页

摘　　要：Microtubules in the axons of nerve cells have unusual stability. To investigate the role of MAP2c in the stabilization of microtubules in neurites, a baculovirus vector has been used to express high levels of MAP2c in insect ovarian Sf9 cells. The cells respond by extending neu-rite-like processes that contain dense bundles of microtubules. The infected cells haveMicrotubules in the axons of nerve cells have unusual stability. To investigate the role of MAP2c in the stabilization of microtubules in neurites, a baculovirus vector has been used to express high levels of MAP2c in insect ovarian Sf9 cells. The cells respond by extending neurite-like processes that contain dense bundles of microtubules. The infected cells have been treated with cold, colchicine and nocodazole, respectively. Results showed that all of the polymers were depolymerized within the first 30 min in cold, while no MT depolymerization was detected after 12 h in colchicine or 6 h in nocodazole. The findings strongly suggest that MAP2c is a factor in conferring drug stability to microtubules in neurons, but factors other than MAP2c or in addition to MAP2c are required for cold stability.

关 键 词：MAP2c MICROTUBULE NEURON neurite. 

分 类 号：Q25[生物学—细胞生物学]