Molecular characterization of diapause hormone in Helicoverpa armigera  

作　　者：Liubin Zhang Weihua Xu Jing Liu Jinliang Shen 

机构地区：[1]Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China [2]Nanjing Agr Univ, Dept Plant Protect, Nanjing 210095, Peoples R China

出　　处：《Chinese Science Bulletin》1999年第6期521-524,共4页

摘　　要：Diapause hormone (DH) is a neurohormone which is secreted from suboesophageal ganglion and responsible for induction of embryonic diapause in many insects. Using rapid amplification of the cDNA ends (RACE) method, the cDNA encoding diapause hormone in Helicoverpa armigera, a main kind of crops pest was cloned. The nucleotide sequence reveals that the mRNA encodes an open reading frame and the 25-aa DH peptide is localized at N-terminal region just after the signal peptide. A homology search showed that H. armigera DH has high homology with the diapause hormone of Bornbyx mori and Helicoverpa zea; and it also belongs to the FXPRL neuropeptide family. Thus, H. armigera DH seems to be a new type of diapause hormone molecule.Diapause hormone (DH) is a neurohormone which is secreted from suboesophageal ganglion and responsible for induction of embryonic diapause in many insects. Using rapid amplification of the cDNA ends (RACE) method, the cDNA encoding diapause hormone inHelicoverpa armigera, a main kind of crops pest was cloned. The nucleotide sequence reveals that the mRNA encodes an open reading frame and the 25-aa DH peptide is localized at N-terminal region just after the signal peptide. A homology search showed thatH. armigera DH has high homology with the diapause hormone ofBombyx mori andHelicoverpa zea; and it also belongs to the FXPRL neuropeptide family. Thus,H. armigera DH seems to be a new type of diapause hormone molecule.

关 键 词：DIAPAUSE HORMONE cDNA CLONING HELICOVERPA armigera. 

分 类 号：Q966[生物学—昆虫学]