NodD binds to target DNA in isologous octamer  

作　　者：刘嵩涛 吕华峰 洪国藩 

出　　处：《Science China(Life Sciences)》1998年第6期592-599,共8页中国科学（生命科学英文版）

基　　金：Pan_DengPlan .Thefirsttwoauthorscontributedequallytothisstudy

摘　　要：NodD, the major regulatory protein of nodulation, was partially purified from Rhizobium leguminosarum 8401(pIJ1518), and its binding sequences within nodF promoter of R.l. bv. viciae were determined by DNase I footprinting. A series of techniques based on gel retardation were used to analyze the NodD_target DNA interaction, showing that NodD binds to target DNA in isologous octamer.NodD, the major regulatory protein of nodulation, was partially purified from Rhizobium leguminosarum 8401(pIJ1518), and its binding sequences within nodF promoter of R.l. bv. viciae were determined by DNase I footprinting. A series of techniques based on gel retardation were used to analyze the NodD_target DNA interaction, showing that NodD binds to target DNA in isologous octamer.

关 键 词：NODD DNase I FOOTPRINTING gel retardation OCTAMER RHIZOBIUM LEGUMINOSARUM bv. viciae . 

分 类 号：Q523[生物学—生物化学]