FOOTPRINTING

作品数：5被引量：1H指数：1
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Mapping conformational changes on bispecific antigen-binding biotherapeutic by covalent labeling and mass spectrometry
《Journal of Pharmaceutical Analysis》2024年第8期1238-1246,共9页Arnik Shah Dipanwita Batabyal Dayong Qiu Weidong Cui John Harrahy Alexander R.Ivanov 
supported by Amgen Inc.,USA and the National Institutes of Health,USA(Grant Nos.:R01CA218500(ARI)and R35GM136421(ARI))。
Biotherapeutic's higher order structure(HOS)is a critical determinant of its functional properties and conformational relevance.Here,we evaluated two covalent labeling methods:diethylpyrocarbonate(DEPC)-labeling and f...
关键词：Covalent labeling/footprinting Liquid chromatography-mass spectrometry Fast photooxidation of proteins Diethylpyrocarbonate Higher order structure characterization 
Local Changes in Chromatin Accessibility and Transcriptional Networks Underlying the Nitrate Response in Arabidopsis Roots被引量：1
《Molecular Plant》2019年第12期1545-1560,共16页Jose M.Alvarez Tomas C.Moyano Tao Zhang Diana E.Gras Francisco J.Herrera Viviana Araus Jose AQ'Brien Laura Carrillo Joaquin Medina Jesus Vicente-Carbajosa Jiming Jiang Rodrigo A.Gutierrez 
This work is funded by Institute Milenio iBio-Iniciativa Cientifica Milenio MINECON,Chile;by grants from the Fondo de Desarrollo de Areas Prioritarias(FONDAP)Center for Genome Regulation(15090007),Chile;Fondo Nacional de Desarrollo Cientifico y Tecnologico(FONDECYT)(1180759),Chile;to R.A.G.J.M.A.is supported by postdoctoral grant FONDECYT(3140336),Chile.J.J.is funded by grant MCB-1412948 from the National Science Foundation,United States;and J.M.by funding from the Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria(INIA),Spain(RTA2015-00014-c02-01).We also want to acknowledge the"Severo Ochoa Program for Centers of Excellence in R&DM from the Agenda Estatal de Investigacion of Spain(SEV-2016-0672(2017-2021))for supporting the scientific services used in this work.
Transcriptional regulation,determined by the chromatin structure and regulatory elements interacting at pro-moter regions,is a key step in plant responses to environmental cues.Nitrate(NO3-)is a nutrient signal that r...
关键词：NITRATE TRANSCRIPTIONAL regulation FOOTPRINTING RNA polymeraseⅡ regulatory networks 
Genome-Wide Identification of Coding Small Open Reading Frames:The Unknown Transcriptome
《Journal of Shanghai Jiaotong university(Science)》2014年第6期663-668,共6页李红梅 胡传圣 白玲 
the National Basic Research Program (973) of China(No.2010CB529205)
The identification of the complete repertoire of functional peptides in a cell is ultimately essential for a systems-wide understanding of its behavior. There have indeed been a plethora of studies purportedly designe...
关键词：small peptides mass spectroscopy ribosome footprinting BIOINFORMATICS 
DNA-protein interaction at erythroid important regulatory elements of MEL cells bv in vivo footprinting
《Chinese Science Bulletin》2000年第12期1101-1108,共8页Jl Xinjun, LIU Depei, XU Dongdong, LI Lei, WANG Jing & LIANG ZhiquanNational Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Faculty of Basic Medical Sciences, Peking Union Medical College, Beijing 100005, China 
Using ligation-mediated PCR method to study the status of DNA-protein interaction at hypersensitive site 2 of locus control Region and βmaj promoter of MEL cell line before and after induction, MEL cell has been cult...
关键词：Β-GLOBIN GENE HS LM-PCR in VIVO FOOTPRINTING MEL cell line. 
NodD binds to target DNA in isologous octamer
《Science China(Life Sciences)》1998年第6期592-599,共8页刘嵩涛 吕华峰 洪国藩 
Pan_DengPlan .Thefirsttwoauthorscontributedequallytothisstudy
NodD, the major regulatory protein of nodulation, was partially purified from Rhizobium leguminosarum 8401(pIJ1518), and its binding sequences within nodF promoter of R.l. bv. viciae were determined by DNase I footpri...
关键词：NODD DNase I FOOTPRINTING gel retardation OCTAMER RHIZOBIUM LEGUMINOSARUM bv. viciae . 
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