Role of calcium in apoptosis of HL-60 cells induced by harringtonine  

作　　者：方敏 张鸿卿 薛绍白 

出　　处：《Science China(Life Sciences)》1998年第6期600-607,共8页中国科学（生命科学英文版）

基　　金：theNationalNaturalScienceFoundationofChina (GrantNos.39730 16 0and 396 70 2 0 4 );theNa tionalEducationalCommissionDoctor’sFoundationofChina

摘　　要：The effect of Ca 2+ on HT induced apoptosis in HL 60 cells was examined. Characteristics of apoptosis induced by harringtonine (HT) or campothecin (CAM) could not be abolished by extracellular calcium chelator EGTA; however, an intracellular Ca 2+ chelator BAPTA AM could block HT or CAM induced HL 60 cell apoptosis. Requirement of intracellular calcium for HT\|induced apoptosis is further supported by the fact that intracellular Ca 2+ depleted HL 60 cells could not undergo HT induced apoptosis. No significant increase of intracellular Ca 2+ was found after HT treatment. By using video enhancement contrast microscopy (VEC), the dynamic changes of intracellular calcium distribution over the whole period of apoptosis in the same individual cell were detected. The results demonstrated the movement of Ca 2+ from cytosol to nucleus after initiation of apoptosis by treatment with HT.The effect of Ca 2+ on HT induced apoptosis in HL 60 cells was examined. Characteristics of apoptosis induced by harringtonine (HT) or campothecin (CAM) could not be abolished by extracellular calcium chelator EGTA; however, an intracellular Ca 2+ chelator BAPTA AM could block HT or CAM induced HL 60 cell apoptosis. Requirement of intracellular calcium for HT\|induced apoptosis is further supported by the fact that intracellular Ca 2+ depleted HL 60 cells could not undergo HT induced apoptosis. No significant increase of intracellular Ca 2+ was found after HT treatment. By using video enhancement contrast microscopy (VEC), the dynamic changes of intracellular calcium distribution over the whole period of apoptosis in the same individual cell were detected. The results demonstrated the movement of Ca 2+ from cytosol to nucleus after initiation of apoptosis by treatment with HT.

关 键 词：APOPTOSIS HARRINGTONINE campothecin CALCIUM ion VIDEO enhancement CONTRAST microscopy. 

分 类 号：Q591[生物学—生物化学]