Quenching of fluorescence in membrane protein by hypocrellin B  

作　　者：乐加昌 庞素珍 

机构地区：[1]Institute of Biophysics, Chinese Academy of Sciences Beijing 100101, China

出　　处：《Science China(Life Sciences)》1997年第2期194-201,共8页中国科学（生命科学英文版）

基　　金：Project supported the National Biological Macromolecule Opening Laboratory and the Opening Laboratory of Photochemistry of the Chinese Academy of Sciences.

摘　　要：The hypocrellin B (HB) was used as a fluorescence quencher to study the basic physical characteris-tics of HB in membrane systems, including the diffusion speed of quencher from aqueous phase into membrane phase, the partition coefficient (P) of quencher between membrane and water, and the fluorescence quenching constant of protein (Ksv; Kq). The experimental results show that the quenching of fluorescence in membrane protein by HB can be determined by the principle of dynamic quenching. The experimental process of fluorescence quenching was ob-served in detail by using the ESR technique. The signal of HB" was found to arise from an electron transfer from ex-cited trytophan to HB.The hypocrellin B (HB) was used as a fluorescence quencher to study the basic physical characteris-tics of HB in membrane systems, including the diffusion speed of quencher from aqueous phase into membrane phase, the partition coefficient (P) of quencher between membrane and water, and the fluorescence quenching constant of protein (Ksv; Kq). The experimental results show that the quenching of fluorescence in membrane protein by HB can be determined by the principle of dynamic quenching. The experimental process of fluorescence quenching was ob-served in detail by using the ESR technique. The signal of HB' was found to arise from an electron transfer from ex-cited trytophan to HB.

关 键 词：FLUORESCENCE QUENCHING MEMBRANE PROTEIN HYPOCRELLIN B. 

分 类 号：Q51[生物学—生物化学]