Subzonal insemination with primary spermatocytes in mouse  

作　　者：Liu, L Sun, QY Duan, CW Liu, H Song, XF Qian, JF Chen, DY 

机构地区：[1]CHINESE ACAD SCI,INST ZOOL,STATE KEY LAB REPROD BIOL,BEIJING 100080,PEOPLES R CHINA

出　　处：《Chinese Science Bulletin》1997年第15期1304-1308,共5页

摘　　要：IT is well known that oocytes, in general condition, can be penetrated by sperm rather thanround spermatids. In 1993, Ogura injected round spermatid nuclei into hamster oocytes anddemonstrated that the spermatid nuclei could transform into pronuclei with DNA synthesis andthat their chromosomes mingled with oocyte chromosomes immediately. In order to avoid themechanical damage to the oocytes, an alternative method, cell-to-cell fusion, was used. Ap-proximately 20%—40% and 30% of round spermatids could be fused with oocytes by

关 键 词：PRIMARY SPERMATOCYTE subzonal INSEMINATION ELECTROFUSION mouse. 

分 类 号：Q492[生物学—生理学]