Aminoacylase from pig kidney contains no disulfide bonds  

作　　者：王洪睿 张彤 王泽峰 王希成 周海梦 

机构地区：[1]Department of Biological Science & Biotechnology,Tsinghua University,Beijing 100084,China National Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China

出　　处：《Science China Chemistry》1995年第12期1448-1454,共7页中国科学（化学英文版）

摘　　要：Both non-reduced/reduced(NR/R)two-dimensional diagonal SDS-PAGE and NR/Rone-dimensional SDS-PAGE showed no disulfide bonds in aminoacylase from pig kidney.Eight andfour thiol groups were modified in the native enzyme by 2-chloromercuri-4-nitrophenol(MNP)andEllman’s reagent,5,5’-dithiobis(2-nitrobenzoic add)(DTNB),and another two and six thiol groupscould be exposed and modified in 7mol/L guanidine hydrochloride,respectively.The enzyme denaturedwith guanidine or urea was found to contain a total of ten thiol groups.This is in good agreement with therecently deduced amino acid sequence from cloned cDNA.It is therefore clear that no disulfide bridges existin aminoacylase from pig kidney.Both non-reduced/reduced(NR/R)two-dimensional diagonal SDS-PAGE and NR/R one-dimensional SDS-PAGE showed no disulfide bonds in aminoacylase from pig kidney.Eight and four thiol groups were modified in the native enzyme by 2-chloromercuri-4-nitrophenol(MNP)and Ellman's reagent,5,5'-dithiobis(2-nitrobenzoic add)(DTNB),and another two and six thiol groups could be exposed and modified in 7mol/L guanidine hydrochloride,respectively.The enzyme denatured with guanidine or urea was found to contain a total of ten thiol groups.This is in good agreement with the recently deduced amino acid sequence from cloned cDNA.It is therefore clear that no disulfide bridges exist in aminoacylase from pig kidney.

关 键 词：AMINOACYLASE DISULFIDE bonds two-dimensional DIAGONAL SDS-PAGE CYSTEINE THIOL group. 

分 类 号：Q55[生物学—生物化学]