c-myc和k-ras基因多态性与辐射诱发小鼠胸腺淋巴瘤的关联性分析  

Analysis on relevance between polymorphism of c-myc and k-ras gene and thymic lymphomas of mice induced by ionizing radiation

作　　者：于雷[1] 刘永哲[1] 孙世龙[1] 杨湘山[1] 武宁[1] 宋祥福[1] 鞠桂芝[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2009年第2期296-298,397,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30670630)

摘　　要：目的:探讨c-myc和k-ras基因多态性与辐射诱发小鼠胸腺淋巴瘤的关系。方法:采用60Co(剂量率0.382 Gy·min-1,总剂量7Gy)分别照射C57BL/6J和BALB/c小鼠建立小鼠胸腺淋巴瘤模型,6个月后处死小鼠,取胸腺组织,用PromegaDNA纯化试剂盒提取胸腺淋巴瘤细胞基因组DNA,以聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)方法对c-myc基因rs51048361(C/T)、k-ras基因rs30221756(A/G)和rs30161609(T/C)单核苷酸多态性进行检测。结果:60Co照射引起2种小鼠的胸腺瘤发生率不同,对应的rs51048361位点基因型分别为C/C和T/T,rs30221756位点基因型分别为A/A和G/G,rs30161609位点基因型分别为T/T和C/C。结论:辐射敏感性不同的C57BL/6J和BALB/c小鼠60Co照射后rs51048361、rs30221756和rs30161609位点基因型不同,推测c-myc和k-ras基因多态性与辐射诱发胸腺淋巴瘤有关联。Objective To investigate the relation between polymorphism of c-myc and k-ras gene and thymic lymphomas of mice induced by ionizing radiation. Methods The thymic lymphomas models of mice were made by ^60Co（dose rate： 0.382 Gy·min^-1, total dose： 7Gy） exposuring to C57BL/6J and BALB/c, 6 months later, the mice were killed and thymus tissues were taken, genome DNA of thymic lymphomas cell was extracted by Promega DNA purification kit, PCR-restriction fragment length polymorphism （RFLP） was used to examine the single nucleotidepolymorphism （SNP） of rs51048361 （C/T） of c-myc gene, rs30221756 （A/G） and rs30161609 （T/ C） of k-ras gene. Results The thymic lymphomas incidence induced by ^60Co exposuring in two kinds of mice was different, the genotypes of rs51048361 site were C/C and T/T, the genotypes of rs30221756 site were A/A and G/ G, the genotypes of rs30161609 site were T/T and C/C. Oonclusion After ^60Co exposuring, the genotypes of rs51048361, rs30221756 and rs30161609 in C57BL/6J and BALB/c with different radiosusceptibility are different, so it is inferred that there is relevance between the polymorphism of c-myc and k-ras gene and thymic lymphomas induced by ionizing radiation.

关 键 词：胸腺淋巴瘤 多态性 单核苷酸 基因 MYC 基因 RAS 

分 类 号：Q345[生物学—遗传学] R733[医药卫生—肿瘤]