乳腺癌患者CYP1A1基因MspⅠ酶切位点多态性的分析  

Analysis of polymorphism of CYP1A1-MspⅠ gene in patients with breast cancer

作　　者：白淑芬[1,2] 杨立新[2] 张爱臣[1] 冷维春[1] 

机构地区：[1]吉林大学中日联谊医院妇产科,吉林长春130033 [2]江苏省南京市第二医院妇产科,江苏南京210003

出　　处：《吉林大学学报（医学版）》2009年第2期377-380,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅基金资助课题(20020644)

摘　　要：目的:探讨细胞色素CYP1A1基因MspⅠ酶切位点多态性与乳腺癌遗传易感性的关系。方法:应用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)分析技术,检测160例乳腺癌患者(病例组)和124例同期住院非乳腺癌患者(对照组),CYP1A1基因MspⅠ酶切位点多态性的3种基因型及等位基因的频数分布。结果:在病例组CYP1A1基因MspⅠ酶切位点基因型频数分布为:TT(45.0%)、TC(46.3%)、CC(8.8%),等位基因频数分布为T(68.1%)、C(31.9%);在对照组CYP1A1基因MspⅠ酶切位点基因型的频数分布为TT(61.3%)、TC(35.5%)、CC(3.2%),等位基因频数分布为T(79.0%)、C(21.0%)。各个基因型在两组中所占的比例差异无显著性(P>0.05);两组T、C等位基因频数比较差异有显著性(P<0.05)。结论:CYP1A1基因MspⅠ酶切位点多态性可能与本地区乳腺癌易感性有关。Objective To investigate the association between CYP1A1-Msp I gene polymorphism and genetic susceptibility of breast cancer. Methods CYP1AI-Msp I gene polymorphism in 160 patients with breast cancer and 124 controls were detected by PCR-RFLP. The frequencies of genotype and allele were analyzed. Results The distribution frequencies of genotype of CYP1A1-Msp I site in case group were TT 45.0%, TC46.3%, CC8.8%, and the frequencies of allelic gene were T （68.1%） and C （31.9%） . While the distribution frequences of genotype of CYP1A1-Msp I site in control group were TT61.3%, TC35.5%, CC3.2%, and the frequences of allelie gene were T79.0%, C21.0%. The ratios of genotypes in each group had no significant difference （P〉0.05）; The frequencies of allelic gene of T and C in each group had significant difference （P〉0.01）. Conclusion CYP1A1-Msp I gene polymorphism may be linked with genetic susceptibility of breast cancer in this area.

关 键 词：乳腺肿瘤 细胞色素P450 CYP1A1 多态性 单核苷酸 疾病遗传易感性 

分 类 号：R737.9[医药卫生—肿瘤] Q75[医药卫生—临床医学]