耐酸耐糖高产乙醇单倍体酵母菌株的选育  

THE SCREENING OF HIGH-ETHANOL-PRODUCING HAPLOIDY YEAST STRAINS TOLERANT TO SUGAR AND ACID

作　　者：何秀良[1] 段俊英[1] 柴明[1] 鞠京丽[1] 蔡崇光[1] 裴永娜 

机构地区：[1]中国科学院沈阳应用生态所 [2]沈阳药学院

出　　处：《微生物学杂志》1989年第3期50-54,60,共6页Journal of Microbiology

摘　　要：在收集大量酵母菌株的基础上,用废糖蜜作碳源,以测定残糖及乙醇产量为指标,经单孢子诱变得到两株活性高,发酵速度较快的稳产、高产酿酒酵母菌株(Saccharomyces cerevisiae)Z—B—4—M—88和Z—S—B—M—33。前者是耐酸菌,在pH4.0,15％糖度下乙醇产量达62.87mg/ml,比原始菌乙醇产量提高了11.2％;后者是耐糖菌,在20％糖度下乙醇产量为70.24mg/ml比原始菌的乙醇量提高11.9％。With the waste molasses as the sole carbon source and the amount of residual sugar and ethanol productivity selected as the screening item, two stable and high ethanol-producing Saccharomyces cerevisiae strains Z-B-4-M-88 and Z-S-B-M-33 were obtained. These two mutants possess high ethanolproducing activity and fast growing in the ethanol fermentation. The former is acid-tolerant in the conditions of pH4.0 and 15% sugar, the ethanol production is 62.87mg/ml which is 11.2% higher than that of the parental strains; the latter is sugar-tolerant and in the conditions of pH4.5 and 20% sugar the ethanol production is 70.24mg/ml which is 11.9% higher than that of the parental strain.

关 键 词：乙醇产量 酵母菌株 原始菌 耐酸菌 发酵速度 糖度计 乙醇发酵 CEREVISIAE SACCHAROMYCES 单孢子 

分 类 号：Q93[生物学—微生物学]