HUMAN CHROMOSOME BANDING WITH RESTRICTION ENDONUCLEASES HaeIII,HinfI AND PstI  

作　　者：张思仲 董伟峰 

机构地区：[1]Department of Medical Genetics, West China University of Medical Sciences, Chengdu 610044, PRC [2]Department of Medical Genetics, West China University of Medical Sciences, Chengdu 610044, PRC Human chromosome banding was carried out with restriction endonucleases (REs)HaeⅢ, HinfⅠ, PstⅠand the effects of some factors on it were examined. HaeⅢinduced Cand G banding patterns, HinfⅠinduced negative C bands but the centromeric heterochro-matin of chromosomes 3 and 4 remained selectively dark-stained. A new heteromorphismof C banded region was discovered in chromosome 4. PstⅠinduced G-like banding pattern.The duration of enzymatic digestion, the concentration of glycerol in the reaction buffer,as well as the ageing and heating of chromosome preparations all had a significant influ-ence on the banding effects of the REs.

出　　处：《Science China Chemistry》1989年第11期1350-1360,共11页中国科学（化学英文版）

基　　金：Project supported by National Natural Science Foundation of China.

摘　　要：Human chromosome banding was carried out with restriction endonucleases (REs)HaeⅢ, HinfⅠ, PstⅠ and the effects of some factors on it were examined. HaeⅢ induced Cand G banding patterns, HinfⅠ induced negative C bands but the centromeric heterochro-matin of chromosomes 3 and 4 remained selectively dark-stained. A new heteromorphismof C banded region was discovered in chromosome 4. PstⅠ induced G-like banding pattern.The duration of enzymatic digestion, the concentration of glycerol in the reaction buffer,as well as the ageing and heating of chromosome preparations all had a significant influ-ence on the banding effects of the REs.Human chromosome banding was carried out with restriction endonucleases (REs)HaeⅢ, HinfⅠ, PstⅠ and the effects of some factors on it were examined. HaeⅢ induced Cand G banding patterns, HinfⅠ induced negative C bands but the centromeric heterochro-matin of chromosomes 3 and 4 remained selectively dark-stained. A new heteromorphismof C banded region was discovered in chromosome 4. PstⅠ induced G-like banding pattern.The duration of enzymatic digestion, the concentration of glycerol in the reaction buffer,as well as the ageing and heating of chromosome preparations all had a significant influ-ence on the banding effects of the REs.

关 键 词：RESTRICTION ENDONUCLEASES HUMAN CHROMOSOME BANDING 

分 类 号：O6[理学—化学]