supported by the Beijing Xisike Clinical Oncology Research Foundation (China) (No.Y-HR2020MS-0156 to T.M.);the National Natural Science Foundation of China (No.82273130 to T.M.).
DNA exonucleases and endonucleases are key executors of the genome during many physiological processes.They generate double-stranded DNA by cleaving damaged endogenous or exogenous DNA,triggering the activation of the...
The CRISPR-Cas system,an adaptive immunity system in prokaryotes designed to combat phages and foreign nucleic acids,has evolved into a groundbreaking technology enabling gene knockout,large-scale gene insertion,base ...
the National Key R&D Program of China(2023YFF1000200);the National Natural Science Foundation of China(31825008 and U21A20276);the Tencent Foundation through the XPLORER PRIZE and the New Cornerstone Science Foundation to Z.H.,and Heilongjiang Touyan Team(HITTY-20190034 to Z.H.).
CRISPR-Cas systems and IS200/IS605 transposon-associated TnpBs have been utilized for the development of genome editing technologies.Using bioinformatics analysis and biochemical experiments,here we present a new fami...
funded by grant no.031B0548 in the frame of the program"Crop plants of the future"from the Bundesministerium fur Bildung und Forschung to A.T.;funded by the Investissement d’Avenir program of the French National Agency of Research for the project GENIUS(ANR-11-BTBR-0001_GENIUS).
In plants and mammals,non-homologous end-joining is the dominant pathway to repair DNA doublestrand breaks,making it challenging to generate knock-in events.In this study,we identified two groups of exonucleases from ...
RNA-guided DNA endonucleases are broadly associated with and play critical roles in prokaryotic adaptive immunity-based CRISPR-Cas systems and IS200/605 family transposons.The Feng Zhang lab recently elucidated the ph...
supported by the National Major Project for Production of Transgenic Breeding(2016ZX08007003)。
Transgenic ruminants are a valuable resource for both animal breeding and biomedical research.The development of transgenic breeding is proceeding slowly,because it suffers from low efficiency of gene transfer and pos...
Supported by the National Natural Science Foundation of China(Nos.21725503,21575038);the Young Top-notch Talent for Ten Thousand Talent Program of China.
The system of clustered regularly interspaced short palindromic repeats(CRISPR)and CRISPR-associated endonucleases(Cas)have been widely used in gene editing,disease treatment,molecular diagnosis and chromosome imaging...
The ability to introduce precise genomic modifications in human cells has profound implications for both basic and applied research in stem cells, ranging from identification of genes regulating stem cell self-renewal...
Supported by the National Natural Science Foundation of China (Grant No. 20672012);Beijing Natural Science Foundation (Grant No. 20822020)
Aqueous nanoparticle suspension of fullerene and its derivatives are currently attracting much attention. To determine the effects of aqueous nanoparticle suspension of a mono-methanophosphonate fullerene and bis-meth...