TRANSPOSASE

作品数：14被引量：20H指数：2
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相关领域：生物学农业科学更多>>
相关机构：江南大学更多>>
相关期刊：《aBIOTECH》《Zoological Research》《Journal of Animal Science and Biotechnology》《Science China(Life Sciences)》更多>>
相关基金：国家自然科学基金国家重点基础研究发展计划国家高技术研究发展计划更多>>
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Innovative genome editing in plants: a transposase and CRISPR combination approach
《Science China(Life Sciences)》2025年第3期893-894,共2页Hamza Sohail Iqra Noor Xuehao Chen Xiaodong Yang 
supported by Jiangsu specially-appointed professor fund(337060046);the Yangzhou University Startup Fund(137012599).
Transgenic plants are extensively utilized in research,enabling scientists to explore gene functions in controlled environments,leading to crops with higher productivity and greater resistance to biotic and abiotic st...
关键词：integration INSERTION CROPS 
CRISPR beyond:harnessing compact RNA-guided endonucleases for enhanced genome editing
《Science China(Life Sciences)》2024年第12期2563-2574,共12页Feizuo Wang Shengsheng Ma Senfeng Zhang Quanquan Ji Chunyi Hu 
The CRISPR-Cas system,an adaptive immunity system in prokaryotes designed to combat phages and foreign nucleic acids,has evolved into a groundbreaking technology enabling gene knockout,large-scale gene insertion,base ...
关键词：CRISPR-Cas OMEGA(ω)system TRANSPOSASE gene-editing compact editors protein engineering miniature Cas effector 
Genome editing in plants using the TnpB transposase system被引量：1
《aBIOTECH》2024年第2期225-230,共6页Qi Li Yongqiang Wang Zhuoting Hou Hang Zong Xuping Wang Yong EZhang Haoyi Wang Haitao Chen Wen Wang Kang Duan 
The widely used clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated nuclease(Cas)system is thought to have evolved from IS200/IS605 transposons.TnpB proteins,encoded by one type of IS20...
关键词：ISAam1 ISDra2 ISYmu1 Plant genome editing Rice TnpB transposon endonuclease 
Review and Evaluate the Bioinformatics Analysis Strategies of ATAC-seq and CUT&Tag Data
《Genomics, Proteomics & Bioinformatics》2024年第3期11-27,共17页Siyuan Cheng Benpeng Miao Tiandao Li Guoyan Zhao Bo Zhang 
supported by the National Institutes of Health,USA(Grant Nos.R35GM142917 to Bo Zhang,U24ES026699 to Bo Zhang,R25DA027995 to Bo Zhang,R03AG070474 to Guoyan Zhao,R01NS123571 to Guoyan Zhao,U19NS130607 to Guoyan Zhao,and U24HG012070 to Bo Zhang and Guoyan Zhao);National Institutes of Health,USA.
Efficient and reliable profiling methods are essential to study epigenetics.Tn5,one of the first identified prokaryotic transposases with high DNA-binding and tagmentation efficiency,is widely adopted in different gen...
关键词：Tn5 transposase EPIGENETIC BIOINFORMATICS ATAC-seq CUT&Tag 
Improvement in Tol2 transposon for efficient large-cargo capacity transgene applications in cultured cells and zebrafish(Danio rerio)被引量：1
《Zoological Research》2024年第3期567-574,共8页Peng-Cheng Wang Hao Deng Rang Xu Jiu-Lin Du Rongkun Tao 
supported by the National Science and Technology Innovation 2030 Major Projects(2021ZD0202200);National Natural Science Foundation of China(32171090,81970264);Shanghai Science and Technology Commission(21ZR1482600);2023 Youth Innovation Promotion Association CAS。
Most viruses and transposons serve as effective carriers for the introduction of foreign DNA up to 11 kb into vertebrate genomes.However,their activity markedly diminishes with payloads exceeding 11 kb.Expanding the p...
关键词：ZEBRAFISH Tol2 transposase TRANSGENE Large payload Synthetic biology 
Hi-Tag:a simple and efficient method for identifying protein-mediated long-range chromatin interactions with low cell numbers
《Science China(Life Sciences)》2024年第5期1027-1034,共8页Xiaolong Qi Lu Zhang Qiulin Zhao Peng Zhou Sai Xian Zhang Jingjin Li Zhuqing Zheng Yue Xiang Xueting Dai Zhe Jin Yaobang Jian Xinyun Li Liangliang Fu Shuhong Zhao 
supported by the National Natural Science Foundation of China(32221005);the Earmarked Fund for CARS(CARS-35);the National Natural Science Foundation of China Outstanding Youth(32125035);Major Project of Hubei Hongshan Laboratory(2021hszd003)。
Protein-mediated chromatin interactions can be revealed by coupling proximity-based ligation with chromatin immunoprecipitation.However,these techniques require complex experimental procedures and millions of cells pe...
关键词：Hi-Tag chromatin interactions transposase tagmentation chromatin proximity ligation low cell numbers 
Construction of a transposase accessible chromatin landscape reveals chromatin state of repeat elements and potential causal variant for complex traits in pigs
《Journal of Animal Science and Biotechnology》2023年第2期529-545,共17页Tao Jiang Ziqi Ling Zhimin Zhou Xiaoyun Chen Liqing Chen Sha Liu Yingchun Sun Jiawen Yang Bin Yang Jianzhen Huang Lusheng Huang 
supported by National Key Research and Development Program of China(2021YFF1000601).
Background:A comprehensive landscape of chromatin states for multiple mammalian tissues is essential for elucidating the molecular mechanism underlying regulatory variants on complex traits.However,the genome-wide chr...
关键词：Chromatin accessibility Pig Tissue specific TRANSCRIPTOME Transposable elements 
A domesticated Harbinger transposase forms a complex with HDA6 and promotes histone H3 deacetylation at genes but not TEs in Arabidopsis被引量：2
《Journal of Integrative Plant Biology》2021年第8期1462-1474,共13页Xishi Zhou Junna He Christos N.Velanis Yiwang Zhu Yuhan He Kai Tang Mingku Zhu Lisa Graser Erica de Leau Xingang Wang Lingrui Zhang W.Andy Tao Justin Goodrich Jian-Kang Zhu Cui-Jun Zhang 
This work was supported by grants from the National Key Research and Development Program of China(2020YFE0202300);the Central Public-interest Scientific Institution Basal Research Fund,the BBSRC under the Grant Reference BB/P008569/1 to J.G.C.N.V.and E.dL.,and an Erasmus plus training award to L.G.
In eukaryotes,histone acetylation is a major modification on histone N-terminal tails that is tightly connected to transcriptional activation.HDA6 is a histone deacetylase involved in the transcriptional regulation of...
关键词：DEACETYLATION FLOWERING Harbinger HDA6 histone acetylation protein complex SANT 
Structural basis of a Tn7-like transposase recruitment and DNA loading to CRISPR-Cas surveillance complex被引量：2
《Cell Research》2020年第2期185-187,共3页Beibei Wang Wenhao Xu Hui Yang 
Supported by the National Natural Science Foundation of China(31971135)to H.Y.
Dear Editor,Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)and CRISPR-associated(Cas) systems confer RNA-guided adaptive immunity against invading genetic elements in prokar yotic cells.1 These syste...
关键词：CRISPR DNA LOADING 
Prokaryotic expression of goldfish Tgf2 transposase with optimal codons and its enzyme activity
《Aquaculture and Fisheries》2019年第3期98-104,共7页Xi Zhao Ruirui Si Mingjuan He Xiayun Jiang Shuming Zou 
This work was supported by the National Science Foundation of China(31572220,31272633,31201760);the National High Technology Research and Development Program of China(863 Program)(2011AA100403);the Shanghai University Knowledge Service Platform(ZF1206).
Tgf2 transposase(Tgf2-TPase),a hAT transposase from goldfish,plays an important role in fish transgenic applications.Previously,the production of the recombinant Tgf2-TPase protein required rigorous fermentation at lo...
关键词：Codon optimization Enzyme activity Prokaryotic expression Tgf2 transposase 
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