北京鸭线粒体基因组全序列测定和分析  

Complete Sequence Determination and Analysis of Beijing Duck Mitochondrial Genome

作　　者：涂剑锋[1,2] 黄银花[1] 刘三凤[3] 李宁[1] 

机构地区：[1]中国农业大学农业生物技术国家重点实验室,北京100094 [2]中国农业科学院特产研究所,吉林132109 [3]江西农业大学动物科技学院,南昌330045

出　　处：《动物学杂志》2009年第2期28-33,共6页Chinese Journal of Zoology

基　　金：国家重点基础研究发展计划项目(No.2006CB200100)

摘　　要：线粒体DNA作为遗传标记,已在家鸡(Gallus gallus)和家鹅(Anser anser)的研究中取得了重大进展,而对家鸭(Anas platyrhychos domesticus)的研究却很少。本研究参照近源物种线粒体基因组序列设计15对引物,通过PCR扩增、测序、拼接,获得北京鸭(A.platyrhychos)线粒体基因组全序列,初步分析其特点和各基因的定位。结果显示,北京鸭线粒体基因组全长16 604 bp,碱基组成为29.19%A、22.20%T、15.80%G、32.81%C,包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个非编码控制区(D-loop),基因组成及排列顺序与其他鸟类相似。基于线粒体D-loop区全序列,用N-J法构建了7种雁形目鸟类系统进化树,结果表明,北京鸭与绿头鸭(A.platyrhychos)系统进化关系较近。The mitochondrial DNA （mtDNA） has been used as a genetic marker in Gallus gallus and Anser anser, but not in the Anas platyrhychos. In this study, we designed 15 primers by referring to close species. Using PCR amplification, sequencing and assembling, we obtained the complete mitochondrial genome of Beijing Duck （A. platyrhychos）. The entire mitochondrial genome was 16 604 bp in length. It contained 37 genes （13 protein coding genes, 2 rRNA, and 22 tRNA） and a non-coding control region （D-loop）. The composition of the nucleotides is 29.19 % A, 22.20 % T, 15.80 % G, and 32.81% C. The genome organization including gene order resembled to that of other birds. Based on the sequence of D-loop region, the neighbor-joining method was adopted to examine the phylogenetic relationships among 7 Anseriform birds. The results indicated that Beijing duck and Mallard （A. platyrhychos ） had closer phylogenetic relationship.

关 键 词：北京鸭 线粒体基因组 序列分析 系统进化树 

分 类 号：Q951[生物学—动物学]