糖链对蚯蚓纤溶酶纤溶活性的影响  

Effects of Sugar Chains on Fibrinolytic Activity of Earthworm Fibrinogenases

作　　者：武金霞[1,2] 殷鹏[1] 张贺迎[2] 

机构地区：[1]河北大学生命科学学院 [2]河北大学生物工程技术研究中心,河北保定071002

出　　处：《中国医药工业杂志》2009年第4期261-263,共3页Chinese Journal of Pharmaceuticals

基　　金：河北省自然科学基金项目(C2005000118)

摘　　要：应用以大豆胰蛋白酶抑制剂(SBTI)为配基的亲和色谱从赤子爱胜蚓中分离了蚯蚓纤溶酶,亲和介质Sepharose-4B对蚯蚓纤溶酶的吸附量为0.04mg/g;以糖苷酶A去除固定在SBTI上的蚯蚓纤溶酶的糖链。辣根过氧化物酶标记的刀豆凝集素(ConA-HRP)亲和印迹和Schiff's染色结果表明,蚯蚓纤溶酶的糖链已基本去除,去除糖链后蚯蚓纤溶酶的活性降低约30%。Earthworm fibrinogenases were separated from Eiseniafoetida using affinity chromatography with soybean trypsin inhibitor （SBTI） as Iigands. The adsorption capacity of Sepharose-4B to earthworm fibrinogenases was 0.04 mg/g. The earthworm fibrinogenases immobilized on SBTI were deglycosylated by glycosidase A. The results of affinity blotting with ConA-HRP and Schiff＇s reagent staining showed that the sugar chains of earthworm fibrinogenases were basically removed, and the fibrinolytic activity reduced about 30 %.

关 键 词：蚯蚓纤溶酶 大豆胰蛋白酶抑制剂 糖苷酶A 糖链 

分 类 号：Q513.2[生物学—生物化学]