脉冲Nd：YAG激光对口腔溃疡组织中NO及NOS含量的影响  

Effect of different energy levels of pulsed Nd∶YAG laser irradiation on contents of nitric oxide and nitric oxide synthase in rat oral ulcer tissues

作　　者：杨苏平[1] 刘鲁川[1] 刘娜[1] 贾文东 

机构地区：[1]第三军医大学大坪医院野战外科研究所口腔科,重庆400042 [2]兰州军区司令部门诊部口腔科,兰州730000

出　　处：《第三军医大学学报》2009年第8期703-705,共3页Journal of Third Military Medical University

基　　金：重庆市科委科技基金(2001-54-21)~~

摘　　要：目的探讨脉冲Nd∶YAG激光不同照射剂量对大鼠实验性口腔黏膜溃疡组织中一氧化氮(NO)及一氧化氮合酶(NOS)含量的影响。方法健康Sprague-Dawley大鼠56只,随机分为7组(n=8):1组为正常对照组,其余6组建立口腔黏膜溃疡动物模型,分别为溃疡组,药物组和激光含量1.5 W40 mJ组、1.5 W 60 mJ组、1.5 W 80 mJ组、1.5 W 100mJ组。末次治疗24 h后观察溃疡愈合时间。采用分光光度仪测定大鼠口腔黏膜组织中NO、NOS的含量。结果实验剂量范围内,1.5 W60 mJ、1.5 W80 mJ激光组较溃疡组NO、NOS活性显著降低(P<0.05)。1.5 W60 mJ、1.5 W80 mJ激光组与正常对照组NO、NOS活性含量没有显著性差异(P<0.05)。结论适合的剂量参数,采用适量脉冲Nd∶YAG激光照射口腔黏膜溃疡能有效降低组织中NO、NOS活性含量,起到治疗口腔黏膜溃疡的作用。Objective To observe the effect of different energy levels of pulsed Nd ： YAG laser irradiation oll the changes of NO and NOS levels in ulcer tissue of oral ulcer model. Methods Forty-eight Sprague-Dawley rats were inflicted with oral ulcer, and then randomly and equally assigned to 6 groups, that is, oral ulcer group, 1% iodine glycerin group and the rest 4 groups receiving pulsed Nd ： YAG laser irradiation at 1.5 W 40 mJ, 1.5 W60 mJ, 1.5 W80 mJ or 1.5 W 100 mJ （once per day, for3 consecutive days）. The other 8 rats served as normal control. In 24 h after last treatment, 4 rats from each group were sacrificed and their cheek pouches were taken out for NO and NOS levels in the mucosal tissue by spectrophotomentry. Ulcer healing were observed 24 h after the last treatment for 8 consecutive days. The efficiency of Nd ： YAG laser irradiation at different powers were evaluated. Results Irradiation groups had significantly shorter healing time com- pared with iodine glycerin group. Level of NO and NOS activity in 1.5 W 60 mJ and 1.5 W 80 mJ groups was evidently lower than the ulcer group （P 〈 0.05） ; but the changes of NO and NOS activity had no significant difference （P 〈0. 05） between the 2 groups and normal control. Conclusion Proper energy of pulsed Nd ： YAG laser irradiation has therapeutic effect on oral ulcer and decreases the NO activity and NOS content, and it is an effective approach to oral cavity mucosa ulcer.

关 键 词：脉冲ND:YAG激光 照射 口腔溃疡 NO NOS 

分 类 号：R776.1[医药卫生—眼科] R781.05[医药卫生—临床医学]