低能氮离子注入诱变选育乳链菌肽产生菌的研究  

Study on Mutagenesis of Nisin-producing Strain Induced by Low Energy N^+ Implantation

作　　者：陈洪卫[1] 陈林海[1] 张国只[1] 

机构地区：[1]郑州大学河南省离子束生物工程重点实验室,河南郑州450052

出　　处：《食品科学》2009年第7期177-180,共4页Food Science

基　　金：国家"973"计划项目(2004CB719604)

摘　　要：本实验以低能氮离子和紫外线作为诱变手段对乳链菌肽产生菌进行诱变,筛选出一株优良菌株Lactococcus lactisK25,发酵效价由初始的1732.45IU/ml提高到2291.75IU/ml,提高了32.3%。遗传稳定性实验结果表明,Lactococcus lactisK25遗传性状稳定良好。In this study, the nisin-producing strain Lactococcus lactis J7 was mutagenized using low energy N^＋ implantation and UV radiation, and its mutant strain Lactococcus lactis K25 was obtained. The nisin potency is increased from 1732.45 IU/ ml to 2291.75 IU/ml, by 32.3% through the mutagenesis ofLactococcus lactis J7. Moreover, Lactococcus lactis K25 shows nice genetic stability.

关 键 词：乳链菌肽 离子束注入 诱变 

分 类 号：Q939.97[生物学—微生物学]