脉冲安培检测-高效阴离子色谱对药品和保健食品中牛磺酸的快速测定  

Quick Determination of Taurine in Drug and Health Food by Pulse Ampere Detection-High Performance Anion Exchange Chromatography

作　　者：邹晓莉[1] 黎源倩[1] 曾红燕[1] 张婧[1] 郑波[1] 

机构地区：[1]四川大学华西公共卫生学院卫生检验教研室,四川成都610041

出　　处：《分析测试学报》2009年第4期470-473,共4页Journal of Instrumental Analysis

基　　金：国家科技支撑计划课题资助项目(07KJB-01)

摘　　要：建立了保健食品中牛磺酸的高效阴离子色谱快速分析方法。以0．25mol／LNaOH溶液作流动相，AminoPAC PA-10（2×250mm）离子色谱柱作分离柱，在Au工作电极，Ag／AgCl为参比电极的脉冲安培检测器上，分离测定了牛磺酸。牛磺酸的线性范围为1．0～50．0mg／L，检出限为0．088mg／L，标准溶液测定的保留时间和峰面积的日内相对标准偏差分别为0．36％和4．8％，日间相对标准偏差为0．89％和5．0％。用该法测定药品和保健食品中的牛磺酸，结果较为满意，测得值相对标准偏差为6．5％，加标回收率为96％～115％。该法无需衍生，具有较高的灵敏度和精密度，6min内可完成1次检测，适合于药品和保健食品中牛磺酸的快速检测。A high performance anion exchange chromatographic method was established for determination of taurine in drug and health food. The sample was directly separated on a Amino PAC PA-10 amino acid-separation column（2 ×250 mm ） and detected by a pulse amperometric detector with Au as working electrode and Ag/AgCl as reference electrode. The calibration curve showed a good linearity for taurine concentration in the range of 1.0 -50. 0 mg/L with a detection limit of 0. 088 mg/L. The intra-day relative standard derivations （RSDs） of retention time and peak area for determination of taurine standards were 0. 36% and 4.8% , respectively, and the inter-day RSDs were 0. 89% and 5.0% , respectively. Satisfactory results were obtained for analysis of drugs and health foods with the proposed method. Recoveries were 96% - 115% and RSD of determination was 6.5%. The analysis procedure could be completely performed in 6 min and without derivation. The results obtained by this method were compared with those by Chinese national standard method （ GB5009. 169 - 2003 ）, and there was no significant difference between two methods. Therefore, the method was rapid, sensitive and accurate, and was suitable for the determination of taurine in drugs and health food sample.

关 键 词：牛磺酸 高效阴离子色谱法 脉冲安培检测 药品 保健食品 

分 类 号：O652.63[理学—分析化学]