蔡氏扶正消癥汤对胃癌细胞体外生长及细胞黏附分子的影响  

Effects of Prescription of Caishi Fuzheng Xiaozheng Decoction on the Proliferation and Cell Adhesion Molecule of Human Gastric Carcinoma Cell Line SGC-7901 in Vitro

作　　者：郎玮[1] 王海波[1] 吴建波[1] 章圣辉[1] 蔡慎初[1] 尹丽慧[1] 

机构地区：[1]温州医学院附属第一医院中医科,浙江温州325011

出　　处：《中华中医药学刊》2009年第1期88-90,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省科技计划资助项目(2008C30010);温州市科技计划项目(Y20070183)

摘　　要：目的:探讨蔡氏扶正消癥汤对人胃癌细胞SGC-7901体外生长及细胞黏附分子的影响。方法:四甲基偶氮唑蓝(3-[4,5-dimethylthiazol-2-y1]-2,5dipheny1tetrazolium bromide,MTT)还原法检测蔡氏扶正消癥汤对SGC-7901细胞增殖的影响;流式细胞仪检测细胞膜表面E-Cadherin,CD44,CD44V6和CD54的表达。结果:蔡氏扶正消癥生药能抑制SGC-7901的增殖。在实验浓度范围内,随着剂量的增加,抑制作用更加明显;同一剂量下,72h内药物作用时间越长,抑制作用越明显。蔡氏扶正消癥汤作用48h后,SGC-7901细胞膜表面的E-Cadherin表达增高,CD44、CD44V6和CD54表达减少。结论:蔡氏扶正消癥汤能够抑制人胃癌细胞SGC-7901的体外增殖,同时能增加E-Cadherin的表达,减少其CD44,CD44V6和CD54的表达,阻止其对邻近正常组织的浸润及远处转移,从而发挥抗癌作用。Objiective ： To investigate the effects of Prescription of Caishi Fuzheng Xiaozheng decoction on the proliferation and cell adhesion molecule changes of human gastric carcinoma cell line SGC-7901 in vitro. Methods ： The proliferation of SGC-7901 cells was measured by MTT colorimetric assay. Flow cytometry was used to analyze the expression of cell adhesion molecules, including E-Cadherin, CD44, CD44V6 and CD54. Results： Caishi Fuzheng Xiaozheng decoction inhibited the proliferation of SGC-7901 cells in a dose-and time-dependent manner. After treated by Caishi Fuzheng Xiaozheng Decoceion for 48h, the expression of E-cadherin increased concomitant with CD44, CD44 V6 and CD54 depressed. Conclusion ： Caishi Fuzheng Xiaozheng decoction plays its anti-cancer function through inhibit proliferation and effect on cell adhesion molecules.

关 键 词：中药方剂 胃癌细胞 E-钙黏蛋白 CD44 CD54 

分 类 号：R730.52[医药卫生—肿瘤] R735.2[医药卫生—临床医学]