Tween80联合42℃温热对人卵巢癌SKOV-3细胞增殖及HSP70蛋白表达的影响  

The effects of Tween 80 in combination with 42℃ hyperthermia on proliferation and protein expression of human ovarian cancer cell line SKOV-3

作　　者：蔡素霞[1] 田晓予[1] 米建强[1] 段文艳[1] 冯磊[1] 王一佳[1] 

机构地区：[1]河南科技大学第一附属医院妇产科,洛阳471003

出　　处：《肿瘤》2009年第4期337-340,共4页Tumor

基　　金：河南省科技攻关计划资助项目(编号:0620304000-1)

摘　　要：目的:观察Tween80联合42℃温热对人卵巢癌SKOV-3细胞热耐受和热休克蛋白(heat shock protein,HSP70)表达的影响。方法:采用MTT法测定Tween80联合42℃温热作用后,SKOV-3细胞的生长抑制率改变;采用免疫细胞化学法测定Tween80联合42℃温热作用后SKOV-3细胞的HSP70表达情况。结果:Tween80联合42℃温热组的SKOV-3细胞生长抑制率较单纯作用组明显升高(P<0.05);Tween80联合42℃重复温热组的细胞增殖率较单纯42℃重复温热组明显下降(P<0.05);不同体积分数(0.025%、0.05%、0.075%和0.1%)的Tween80联合42℃温热组与单纯温热组相比,SKOV-3细胞的HSP70表达均下降,并且随着Tween80浓度增加,HSP70的表达逐渐下降。结论:Tween80联合42℃温热对人卵巢癌SKOV-3细胞的生长抑制可能有相加或协同作用,Tween80可抑制加热后细胞的HSP70表达和热耐受性产生。Objective：To investigate the effects of Tween 80 combined with 42℃ hyperthermia on the thermotolerance and the expression of heat shock protein （HSP70） in the human ovarian cancer cell line SKOV-3. Methods：The inhibition ratio of cell proliferation was detected by MTT assay after treatment with Tween 80 combined with 42℃ hyperthermia. The expression of HSP70 was detected by immunocytochemical method. Results ： The inhibition ratio of proliferation of SKOV-3 ceils was greatly increased and the proliferation ratio was obviously decreased after the combined treatment with Tween 80 and 42℃ hyperthermia compared with that of the Tween 80 or 42℃ hyperthermia single treatment group （both P 〈 0.05 ）. The expression of HSP70 protein was decreased after combined treatment with different concentrations of Tween 80 （0. 025%, 0.05% , 0. 075% , and 0.1% ） plus 42℃ hyperthermia compared with the groups treated with single 42℃ hyperthermia. With the increase in the concentrations of Tween 80, the HSP70 expression was decreased gradually. Conelusion：Tween 80 combined with 42℃ hyperthermia has additive or synergistic effect in inhibiting the proliferation of SKOV-3 cells. Tween 80 can inhibit the expression of HSP70 and thermotolerance of the ceils after hyperthermia.

关 键 词：卵巢肿瘤 热疗 热耐受 热休克蛋白70 免疫细胞化学 吐温80 细胞 SKOV-3 

分 类 号：R737.31[医药卫生—肿瘤] R730.5[医药卫生—临床医学]