葡萄糖氧化酶电极中酶固定化的研究  

Study on immobilization of enzyme in glucose oxidase electrode

作　　者：周文瑜[1] 王宝根[1] 楼一心 张永勤 

机构地区：[1]华东理工大学生物反应器国家重点实验室

出　　处：《中国抗生素杂志》1998年第2期140-143,共4页Chinese Journal of Antibiotics

摘　　要：研究了以丝纤维为载体，三聚氯氰为交联剂固定化葡萄糖氧化酶膜的方法和条件。实验表明，用二氧六环－二甲苯（质量比１１）为三聚氯氰的溶剂，固定化酶膜效果较好。酶的偶联条件以温度２５℃、ｐＨ＝９、酶液浓度４ｍｇ／ｍｌ为最佳，此时固定化酶膜稳定性好，反复使用３０次酶膜活性保留９０％以上，贮存在离子强度较高的溶液中，酶膜的半衰期为４０～５０周。A study was carried out on the immobilized methods of glucose oxidase membrane with carrier of silk fabrics and cross linker of tricyanogen chloride. Experimental results showed that the performance of immobilized enzyme membrane was good by using dioxane-dimethyl benzene (1∶1, W/W) as solvent of tricyanogen chloride. The optimum coupling conditions of enzyme are as follows: T=25℃, pH9 and concentration of enzyme 4mg/ml. Reserving in a liquid with higher ionic strength, the half life of enzyme membrane is 40￣50weeks.

关 键 词：固定化酶 丝纤维 葡萄糖氧化酶 酶电极 

分 类 号：Q532.303[生物学—生物化学]