16S rDNA-RFLP法快速鉴定蒙古国传统乳制品中的乳酸菌  

Rapid Identification of Lactic Acid Bacteria from Mongolia Home-made Fermented Milk Products Rapidly Identified by 16S rDNA-RFLP Method

作　　者：艾日登才次克[1] 于洁[1] 杜晓华[1] 李莉[1] 王炜宏[1] 孙天松[1] 张和平[1] 

机构地区：[1]内蒙古农业大学乳品生物技术与工程教育部重点实验室,呼和浩特010018

出　　处：《中国农业科技导报》2009年第2期63-68,共6页Journal of Agricultural Science and Technology

基　　金：国家863计划项目(2006AA10Z345和2007AA10Z353)资助

摘　　要：以17株标准菌株为参照,采用限制性片段长度多态性(RFLP)分析和16S rDNA序列分析相结合的技术,对分离自蒙古国5个地区传统乳制品中的55株乳酸菌进行了快速鉴定。结果表明,通过限制性内切酶AluⅠ、HaeⅢ、BsmaⅠ、TspRⅠ和HinfⅠ的指纹图谱分析,将49株乳杆菌和2株片球菌准确鉴定到种,其他4株肠球菌鉴定到属。采用16S rDNA-RFLP方法鉴定乳酸菌具有准确性,可靠性和高效性。55 strains of lactic acid bacteria were isolated from traditional homemade fermented milk products in 5 different regions of Mongolia. All strains were identified on basis of restriction fragment length polymorphism （RFLP） analysis and 16S rDNA sequences compared with 17 standard strains. RFLP analysis used a set of restriction enzymes, Alu I , Hae Ⅲ, Bsma I , TspR I and Hinf I . The results showed that 49 Lactobacilli strains and 2 Pdeio- coccus strains were identified to species level, while the other 4 cocci strains were identified at genus level. 16S rDNA-RFLP method was of accuracy, reliability and efficiency for identifying lactic acid bacteria.

关 键 词：乳酸菌 快速鉴定 限制性片段长度多态性(RFLP) 16S RDNA序列分析 

分 类 号：Q93-331[生物学—微生物学]