实验性自身免疫性脑脊髓炎中γδT细胞的变化及血吸虫卵抗原干预对其的影响  

Change of γδ T cells and effect of schistosome egg antigen on it in experimental autoimmune encephalomyelitis

作　　者：蒋建华[1] 董万利[1] 倪健强[1] 尹岭[2] 张金涛[2] 邢广羽[2] 

机构地区：[1]苏州大学附属第一医院神经内科,215006 [2]中国人民解放军总医院神经信息中心,北京市100853

出　　处：《实用医学杂志》2009年第8期1198-1200,共3页The Journal of Practical Medicine

基　　金：江苏省卫生厅医学科技发展基金资助重大课题(编号:H200302)

摘　　要：目的:研究髓鞘蛋白脂质蛋白多肽178-191(PLP178-191)免疫后小鼠体内γδ T细胞比例的变化以及日本血吸虫虫卵抗原(SEA)干预对其的影响。方法:用流式细胞仪检测技术分别观察对照组、PLP178-191免疫组、SEA干预组小鼠肠上皮内CD3+细胞中γδ T细胞百分比的变化。结果:对照组肠上皮内淋巴细胞CD3+细胞中γδ T细胞百分比为(22.95±7.26)%,PLP178-191免疫组γδ T细胞比例明显升高(P<0.01),SEA干预组则较PLP178-191免疫组有所下降(P<0.01)。结论:γδ T细胞与实验性自身免疫性脑脊髓炎(EAE)发病可能有着密切关系。EAE的干预能使γδ T细胞升高幅度有一定程度的降低,这可能是SEA用于EAE治疗研究的机制之一。Objective To determine the change of γδT cells and effect of schistosome egg antigen （SEA） on it in mice immunized by proteolipid protein 178-191 （PLP178-191）. Methods The proportion of γδT cells in CD3＋ T ceils in intestinal epithelium of mice in control group, PLP178-191immune group, and SEA intervention group were detected by flow cytometry. Results There were significant differences in the proportion of γδT cells in CD3＋ T cells between the control group and PLP178-191 immune group [ （22.95 ±7.26）% vs （39.95 ±6.65）%, P 〈 0.01 ） ], and between the PLP178-191 immune group and SEA intervention group [ （39.95 ±6.65）% vs （27.70± 6.97）%, P 〈 0.01）], and between the control group and SEA intervention group [ （22.95 ± 7.26）% vs （27.70 ± 6.97）%, P 〈 0.05） ]. Conclusions γδT cells may contribute to the pathogenesis of experimental autoimmune encephalomyelitis. The administration of SEA can control the increase of γδT cells.

关 键 词：脑脊髓炎 自身免疫性 实验性 蛋白脂蛋白 SJL/J小鼠 ΓΔT细胞 肠上皮内淋巴细胞 

分 类 号：R744.51[医药卫生—神经病学与精神病学]