PTEN基因对人膀胱癌细胞系BIU-87化疗敏感性的影响  

Effect of PTEN gene on chemosensitivity of bladder cancer cell lines BIU-87

作　　者：鲁雄兵[1] 张建华[2] 杨为民[2] 周四维[2] 

机构地区：[1]南昌大学第二附属医院泌尿外科,330006 [2]华中科技大学同济医学院附属同济医院泌尿外科

出　　处：《现代泌尿生殖肿瘤杂志》2009年第2期102-104,109,共4页Journal of Contemporary Urologic and Reproductive Oncology

摘　　要：目的研究抑癌基因PTEN转染对人膀胱癌细胞系BIU-87化疗敏感性的影响。方法将携有PTEN基因的重组真核表达质粒pBp-PTEN转化大肠杆菌DH5α并扩增,抽提纯化质粒并进行酶切鉴定;pBp-PTEN体外转染BIU-87细胞(pBp-PTEN-BIU87),筛选稳定转染的细胞并扩增培养,以转染了空质粒pBp的BIU-87细胞(pBp-BIU87)和正常BIU-87细胞为对照,用RT-PCR检测PTEN的表达情况,应用MTT法分别研究转染前、后膀胱癌细胞对不同浓度抗癌药物丝裂霉素(MMC)、羟基喜树碱和吡柔比星的敏感性变化。结果RT-PCR证实PTEN在转染的BIU-87细胞中能稳定表达。PTEN转染后,BIU-87细胞对MMC的药物敏感性明显提高,对吡柔比星和羟基喜树碱的药物敏感性无明显变化。结论PTEN基因转染能提高人膀胱癌细胞系BIU-87对抗癌药物MMC的敏感性。Objective To study the effect of transfection with antioncogene PTEN on the chemosensitivity against anticancer drugs of human bladder cancer cell lines BIU-87. Methods The eukaryotic expression plasmid containing PTEN（pBp-PTEN）was introduced into E. coli DHSa and amplified. The plasmid pBp-PTEN was prepared and identified by restriction enzyme. BIU-87 was transfected with pBp-PTEN. The positive cell clones（pBp-PTEN-BIU87） were selected and ampli- fied. BIU87 transfected with empty plasmid （pBp-BIU87） and BIU-87 were employed as control groups,and the expression of PTEN was detected by RT-PCR. Chemosensitivity of BIU-87 against mitomycin C（MMC）,hydroxycamptothecin and pirarubicin before and after transfection were measured by MTT assay. Results After transfection with pBp-PTEN,the chemosensitivity of BIU-87 against MMC was increased significantly, but against hydroxycamptotheein and pirarubicin was no change. Conclusions By transfection with PTEN, the chemosensitivity of BIU-87 against MMC was increased.

关 键 词：膀胱肿瘤 转染 PTEN基因 化疗敏感性 

分 类 号：R737.14[医药卫生—肿瘤] R730.264[医药卫生—临床医学]