BCMA基因5'上游序列定点删除突变对启动子活性的影响  

Effect on the promoter activity of BCMA gene 5'-flanking region deletion mutation

作　　者：邓少丽[1] 程小星[2] 蹇锐[2] 蒋静[2] 

机构地区：[1]大坪医院野战外科研究所检验科,重庆400042 [2]基础部微生物学教研室,重庆400038

出　　处：《中国现代医学杂志》2009年第7期1014-1016,共3页China Journal of Modern Medicine

摘　　要：目的探索BCMA基因5'上游序列-24～-26位点删除突变对启动子活性的影响,为进一步研究BCMA基因的表达调控机制提供实验依据。方法设计一对突变引物,采用定点删除技术,删除BCMA基因5'上游序列-24～-26位点,电转J558L细胞检测荧光素酶的表达,观察荧光素酶相对活性。结果成功删除BCMA基因-24～-26"AAA",启动子活性明显下降。结论BCMA基因5'上游序列-24～-26位点可能是RNA聚合酶识别的重要位点,也可能为重要转录因子结合位点。[Objective] To investigate the effect on the promoter activity of BCMA gene 5′-flanking region deletion mutation, and study the regulation mechanism of BCMA expression. [Methods] AAA located at the -26--24 upstream of BCMA gene was deleted by direct deletion technique. The luciferase expressions were observed ＇,after the reporter had been transfected into J558L cells. [Results] AAA located at the -26--24 upstream of BCMA gene was deleted successfully. The BCMA gene promoter activity was decreased obviously. [Conclusions] -26--24 upstream of BCMA gene maybe the important site reeoganized by RNA polymerase, and maybe the eonjugate site of important transcription factor.

关 键 词：BCMA基因 启动子活性 基因突变 

分 类 号：R394[医药卫生—医学遗传学] R459.5[医药卫生—基础医学]