pBV220载体中外源基因高效表达的自动化设计  

Automated Design for High-expression of Exogenous Genes with pBV220 Vector

作　　者：查磊[1,2] 应晓敏[1] 王立贵[1] 曹源[1] 骆志刚[2] 苑波[2] 李伍举[1] 

机构地区：[1]军事医学科学院基础医学研究所计算生物学中心,北京100850 [2]国防科技大学计算机学院,长沙410073

出　　处：《中国生物化学与分子生物学报》2009年第4期379-382,共4页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金资助项目(No.30470411)~~

摘　　要：pBV220载体是国内科学家构建的原核系统表达载体,目前仍在广泛应用.但是,实现外源基因的高效表达需要综合考虑诸如RNA二级结构等多种因素,极其耗时费力.为此,基于我们提出的pBV220载体中外源基因高效表达数学模型,编写了外源基因高效表达的自动化设计软件,并可定性用于原核系统其它载体中外源基因表达水平分析,最终为加快实验进程提供帮助.pBV220 is a widely used prokaryotic vector constructed in China to expressing exogenous genes. As various factors, such as RNA secondary structure, may influence the yielding of a vector system, it is time-and labor-consuming to achieve high-level expressions of exogenous genes. Here we introduced a program to evaluate the gene expression with a pBV220 vector based on our mathematical model previously reported. The program may be used for automatic vector designs and could be potentially applied to other prokaryotic expression vectors to supply predictive information for molecular biologists.

关 键 词：PBV220 高效表达 自动化设计 

分 类 号：Q811.4[生物学—生物工程]