一株降解甲氰菊酯的光合细菌的分离鉴定及其降解酶初步分析  

Isolation,Identification of Fenpropathrin-degrading Strain PSB07-14 and Preliminary Analysis of Its Degradation Crude Enzyme

作　　者：罗源华 张战泓[2] 刘勇[1,3] 张松柏[1,3] 张德咏[1,3] 罗香文 成飞雪 

机构地区：[1]湖南省植物保护研究所,湖南长沙410125 [2]湖南省蔬菜研究所,湖南长沙410125 [3]中南大学研究生院隆平分院,湖南长沙410125

出　　处：《天津农业科学》2009年第2期1-5,共5页Tianjin Agricultural Sciences

基　　金：国家高技术研究发展计划(863)项目(2006AA10Z401);国家"十一五"科技支撑计划项目(2006BAD17B08;2006BAD08A08)

摘　　要：从某农药厂污泥中筛选分离出一株高效降解甲氰菊酯(Fenpropathrin)的光合细菌,研究了其降解特性及生物学特性。根据分离菌株的细胞形态结构、活细胞光吸收特征、生理生化特征及其16S rDNA序列同源性鉴定降解菌;气相色谱法测定该菌降解甲氰菊酯的能力;采用超声波破碎法提取该菌降解粗酶,利用(NH4)2SO4分段盐析并测定酶活性。结果表明:PSB07-14属红假单胞菌属(Rhodopseudomonas sp.);该菌以共代谢方式降解甲氰菊酯,对甲氰菊酯的最高耐受浓度为800mg/L,降解最佳条件为:30～35℃、pH6～7,光照培养15d对600mg/L甲氰菊酯降解率达48.41%。降解酶测定结果表明:30%～60%(NH4)2SO4沉淀的蛋白降解活性最高。A photosynthetic bacterial strain PSB07-14, with degradability of fenpropathrin, was isolated and identified as Rhodopseudomonas sp. based on its morphology, physiology and homology of 16S rDNA sequence. The degrading characteristics showed the optimum conditions of degrading fenpropathfin were 30 - 35 ℃ pH 6 - 7, respectively. This strain could grow in the media supplied with fenpropathrin up to 800 mg/Land degrade fenpropathrin by cometabolic way. The degradation rate of fenpropathrin was up to 48.41% in a concentration of 600 mg/L within 15 d. The degradation crude enzyme was extracted and subsided by （NH4）2SO4, the results of subsiding enzyme activity showed the highest enzyme activity appeared in the subsiding of 30% - 60%（NH4）2SO4.

关 键 词：甲氰菊酯 红假单胞菌 生物降解 降解酶 

分 类 号：X172[环境科学与工程—环境科学]