生物表面活性剂产生菌的筛选及其发酵条件的初步优化  

Screening and Primary Optimizing of Cultural Medium of Biosurfactant Producing Strain

作　　者：梁艳玲[1,2,3] 骆永明[2,3] 刘五星[2,3] 王纯利[1] 李振高[2,3] 

机构地区：[1]新疆农业大学资源与环境学院,乌鲁木齐830052 [2]中国科学院南京土壤研究所土壤环境与污染修复重点实验室,南京210008 [3]土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所),南京210008

出　　处：《土壤》2009年第2期243-247,共5页Soils

基　　金：国家重点基础研究发展规划(973)项目(2002CB410809);国家自然科学基金重点项目(40432005);中国科学院南京土壤研究所知识创新工程领域前沿项目(ISSASIP0724)资助

摘　　要：以石油为C源,经过富集培养从石油污染土壤中筛选出15株具有较强产生物表面活性剂能力的菌株。其中菌株BS-5产生物表面活性剂的能力最强,该菌发酵液的表面张力可达27.3 mN/m(空白发酵液表面张力为54.5 mN/m)。通过红外光谱分析发现该菌产生的生物表面活性剂为糖脂类物质。另外,通过正交试验对该菌的培养基条件进行初步优化,结果以植物油10g/L、MgSO_4 0.2g/L、K_2HPO_4 1.0g/L、KH_2PO_4 1.0g/L、蛋白胨1.0 g/L、FeSO_40.05g/L、CaCl_2 0.02g/L、初始pH值7.5为最佳。通过16S rDNA测序结果表明该菌为铜绿假单胞菌(Pseudomonas aeruginosa)。Biosurfactant producing strains were screened through enrichment from the oil sludge with crude oil as sole carbon source and 15 strains which could produce higher yield of biosurfactant were obtained. Strain BS-5 could produce highest biosurfactant and be able to reduce the surface tension of fermentation broth to 27.3 mN/m （the surface tension of pure water is 71.5 mN/m）. Analytic results by FT-IR showed that the biosurfactant of the strain was a kind of glyeolipid. In addition, the cultural conditions of the strain were primarily optimized by using orthogonal experiment method. The best result was： vegetable oil 10 g/L, MgSO4 0.2 g/L, K2HPO4 1.0 g/L, KH2PO4 1.0 g/L, peptone 1.0 g/L, FeSO4 0.05 g/L, CaCl2 0.02 g/L and initial pH 7.5. The strain was identified as Pseudomonas aeruginosa according to microscope observation and the 16S rDNA gene sequence.

关 键 词：生物表面活性剂 菌株筛选 正交试验 培养条件优化 

分 类 号：X172[环境科学与工程—环境科学]