酶促半合成人胰岛素的副反应及纯化  

Side Reaction and Purification of the Enzyme-catalyzing Semi-synthesis of Human-insulin

作　　者：刘钧松[1] 汤钧[1] 刘莹[1] 李彬[1] 周慧[1] 李惟[1] 卜凤泉[2] 

机构地区：[1]吉林大学分子生物学系,长春130023 [2]白求恩医科大学应用基础医学研究所,长春130021

出　　处：《吉林大学自然科学学报》1998年第1期103-106,共4页Acta Scientiarum Naturalium Universitatis Jilinensis

摘　　要：研究酶促半合成人胰岛素的副反应．采用DEAE－SephadexA－25阴离子交换层析纯化合成人胰岛素中间体衍生物，去除脱八肽胰岛素以及人胰岛素类似物，精制酶促半合成人胰岛素．Human-insulin is the most extensively used drug for curing,diabetes. Up to now, there have been two ways to produce human-insulin: making use of recombinant DNA technology and semisynthesis method, which is catalysed by proteases in specific organic media. The present paper deals with the some problems existed in the semi-synthesis of human-insulin catalysed by trypsin. The mainby-product was identified in the semi-synthesis of human-insulin. The small peak appeared in front of the main peak of human-insulin in RP-HPLC, which showed the same reserve time with the standardDOI. The present paper reports also a simple method for purifying human-insulin prepared by means of semi-synthesis method. The crude semi-synthesis human-insulin was purified by means of DEAESephadex A-25 ion-exchange chroma tography. The results showed that the purified product displayed a single band at the PAGE pattern, the same reserve time as the standard human-insulin in the RPHPLC.

关 键 词：人胰岛素 脱八肽胰岛素 副反应 纯化 酶促反应 

分 类 号：Q578[生物学—生物化学] TQ467.32[化学工程—制药化工]