Biochemical effects of gadolinium chloride in rats liver and kidney studied by ^1H NMR metabolomics  

作　　者：廖沛球 魏来 吴惠丰 李伟生 吴亦洁 李晓晶 倪嘉缵 裴奉奎 

机构地区：[1]Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China [2]Graduate School of the Chinese Academy of Sciences,Beijing 100039,China

出　　处：《Journal of Rare Earths》2009年第2期280-287,共8页稀土学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (20575065,20637010)

摘　　要：The biochemical effects of gadolinium chloride were studied using high-resolution IH nuclear magnetic resonance （NMR） spec- troscopy to investigate the biochemical composition of tissue （liver and kidney） aqueous extracts obtained from control and gadolinium chloride （GdCl3） （10 and 50 mg/kg body weight, intraperitoneal injection, i.p.） treated rats. Tissue samples were collected at 48, 96 and 168 h p.d. after exposure to GdCI3, and extracted using methanol/chloroform solvent system. ^1H NMR spectra of tissue extracts were analyzed by pat- tern recognition using principal components analysis. The liver damages caused by GdCl3 were characterized by increased succinate and decreased glycogen level and elevated lactate, alanine and betaine concentration in liver. Furthermore, the increase of creatine and lactate, and decrease of glutamate, alanine, phosphocholine, glycophosphocholine （GPC）, betaine, myo-inositol and trimethylamine N-oxide （TMAO） levels in kidney illustrated kidney disturbance induced by GdCl3.The biochemical effects of gadolinium chloride were studied using high-resolution IH nuclear magnetic resonance （NMR） spec- troscopy to investigate the biochemical composition of tissue （liver and kidney） aqueous extracts obtained from control and gadolinium chloride （GdCl3） （10 and 50 mg/kg body weight, intraperitoneal injection, i.p.） treated rats. Tissue samples were collected at 48, 96 and 168 h p.d. after exposure to GdCI3, and extracted using methanol/chloroform solvent system. ^1H NMR spectra of tissue extracts were analyzed by pat- tern recognition using principal components analysis. The liver damages caused by GdCl3 were characterized by increased succinate and decreased glycogen level and elevated lactate, alanine and betaine concentration in liver. Furthermore, the increase of creatine and lactate, and decrease of glutamate, alanine, phosphocholine, glycophosphocholine （GPC）, betaine, myo-inositol and trimethylamine N-oxide （TMAO） levels in kidney illustrated kidney disturbance induced by GdCl3.

关 键 词：gadolinium chloride NMR LIVER KIDNEY metabolomics tissue extracts rat rare earths 

分 类 号：R575[医药卫生—消化系统] R692[医药卫生—内科学] R445.2[医药卫生—临床医学]