S-腺苷-L-蛋氨酸高密度发酵工艺优化  

Optimization of high-cell-density fermentation process for S-adenosyl-L-methionine production

作　　者：王杰鹏[1] 韩晋军[1] 李晓楠[1] 刘沛溢[1] 谭天伟[1] 

机构地区：[1]北京化工大学生命科学与技术学院,北京100029

出　　处：《生物工程学报》2009年第4期533-536,共4页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.20876011);国家高技术研究发展计划(863计划)(Nos.2006AA020102;2006AA020103;2006AA020201;2007AA100404);国家重点基础研究发展规划项目(973项目)(Nos.2007CB707804;2007CB714304);北京市自然科学基金(No.2071002);北京市科技计划项目(No.D0205004040211);北京市教育委员会共建项目专项资助~~

摘　　要：利用酿酒酵母在5L发酵罐高密度发酵生产S-腺苷-L-蛋氨酸(SAM)后期存在稳定性差的问题,本研究考察了在补糖中添加磷酸氢二铵、谷氨酸钠、三磷酸腺苷二钠来提高酿酒酵母发酵后期的稳定性。通过4批5L发酵罐高密度流加发酵实验研究发现:在发酵34h左右,菌体干重超过100g/L后,开始添加50克L-蛋氨酸,并在补糖中加入10g/L三磷酸腺苷二钠,发酵65.7h,最高生物量干重达到180g/L,SAM产量达到17.1g/L。Poor stability existed in the anaphase of the high-cell-density fermentation of Saccharomyces crevisiae for S-adenosyl-L- methionine （SAM） production in 5 L fermentor. To improve the fermentation stability, we studied the addition of diammonium hydrogen phosphate, sodium glutamate and adenosine disodium triphosphate into glucose feeding solution. Study of four fed-batch cultures showed that, after 34 h fermentation, when dry cell weight reached 100 g/L, the addition of 50 g pre-L-methionine and glucose feeding with 10 g/L adenosine disodium triphosphate was optimal for SAM production. Under this condition, after 65.7 h fermentation, both the dry cell weight and the yield of SAM reached the maximum, 180 g/L and 17.1 g/L respectively.

关 键 词：S-腺苷-L-蛋氨酸 ATP 高密度发酵 L-蛋氨酸 

分 类 号：TQ922[轻工技术与工程—发酵工程]