麦芽汁、多效唑等因素对大麦组织培养的效应  

A STUDY ON EFFECTS OF MALT EXTRACT,MET AND OTHER FACTORS ON ANTHER CULTURE OF BARLEY

作　　者：杜永芹[1] 陈如梅[1] 张国荣[1] 奏国卫 余立云[1] 黄培忠[1] 马俊虎[1] 周爱华 

机构地区：[1]上海市农业科学院作物育种栽培研究所,上海201106 [2]上海市南汇县种子公司,上海201300

出　　处：《上海农业学报》1998年第2期85-88,共4页Acta Agriculturae Shanghai

基　　金：上海市农业科学院青年科技基金

摘　　要：以不同基因型大麦为材料，就麦芽计、MET及愈伤组织低温处理对大麦花粉离体培养的影响进行了研究。结果表明，在含有10％麦芽汁的诱导培养基上花药反应早，诱导率和绿茵率均有不同程度的提高；分化培养基中添加1．5mg／LMET可明显增强幼苗素质；在本试验条件下，以MET0.5～1.0mg／L＋IBA0．3mg／L配合使用（壮苗生极培养），有利于植株地上部与地下部的协调生长；愈伤组织经低温（5～8℃）保存效果良好，为大麦试管苗越夏提供了一条新途径。The experiments were carried out using different barley genotypes for determining the effects of malt extract,MET and low temperature treatment of callus on antherculture. The results showed that the addition of 6% malt extract to induction medium couldadvance anther' s response and enhance the frequencies of calli and green plants;the additionof 1. 5 mg/L. MET to regeneration medium was useful for improving the quality of greenplants 1 0. 5~ 1. 0 mg/L MET combined with 0. 3 mg/L IBA could promote the growth ofshoots and roots ;storing calli at low temperature had a good effect,it is a new way for ensuring the oversummering of test tube seedlings.

关 键 词：大麦 花药培养 麦芽汁 愈伤组织 壮苗 多效唑 

分 类 号：S512.303.5[农业科学—作物学]