普洱茶提取物对饮食诱导肥胖大鼠脂质合成相关基因表达的影响  

EFFECT OF PU-ERH TEA ON LIPOGENESIS AND EXPRESSION OF RELATIVE GENES IN OBESE RAT FED WITH HIGH FAT DIET

作　　者：袁华兵[1] 钟婕[1] 易娟 赵燕 曹进[1] 

机构地区：[1]中南大学临床药理研究所茶与健康研究室,长沙410078 [2]湖南长沙卫校,410100 [3]Heart & Vascular Institute, College of Medicine, Penn State University, USA

出　　处：《营养学报》2009年第2期167-171,176,共6页Acta Nutrimenta Sinica

摘　　要：目的观察普洱茶提取物(PTE)对饮食诱导性肥胖大鼠脂质合成相关基因的影响,探讨普洱茶抗肥胖的作用机制。方法30只雄性Sprague-Dawley大鼠随机分为3组(n=10):正常对照组,普通饲料;高脂组,高脂饲料;高脂+PTE组,高脂饲料+PTE。采用高脂饲料建立肥胖大鼠模型,测定大鼠体重和脂肪组织重量,判定减肥效果;采用real-timePCR检测PTE对脂质合成相关基因的表达影响。结果PTE能有效减轻动物体重和脂肪组织重量,显著下调二酰基甘油酰转移酶-1(DGAT1)、固醇辅酶A去饱和酶1(SCD1)和固醇调节元件结合蛋白(SREBP)-1cmRNA的表达。结论PTE可通过调节脂质合成相关基因,减少脂肪的合成,达到预防肥胖的作用。Objective To examine the effect of Pu-Erh tea extract （PTE） on genes expression of lipogenesis in white adipose tissue of rats fed high fat diet. Method Thirty male SD rats were randomly divided into three groups （n=10）： the control group （basal diet）; the high fat group （high fat diet）; the PTE group （high fat diet ＋ Pu-Erh tea extract）. Body weight and. adipose tissue were measured. Expression of genes regulating lipid metabolism was assessed in adipose tissUe. Results PTE supplementation prevented diet-induced increases in body weight and adipose tissue. Diacylglycerol acyltransferase-1 （DGAT1）, stearoyl-CoA desalurase-1 （SCD1） and sterol regulatory element binding protein-1c （SREBP-1c） mRNA levels were markedly decreased in adipose tissue of rats fed PTE. Conclusion This study shows for the first time that Pu-Erh tea extract prevents diet-induced obesity, and this effect is partly mediated via a direct influence on adipose tissue.

关 键 词：普洱茶 营养性肥胖 脂质合成 固醇辅酶A去饱和酶1 二酰基甘油酰转移酶-1 

分 类 号：R151.2[医药卫生—营养与食品卫生学]