宫颈癌组织中人乳头瘤病毒16E6基因突变分析  

Human papillomavirus 16E6 gene mutation in cervical carcinoma tissues

作　　者：孙峰[1] 哈小琴[2] 吕同德[2] 邢传平[1] 刘斌[1] 曹晓哲 

机构地区：[1]兰州军区兰州总医院病理科,甘肃兰州730050 [2]兰州军区兰州总医院医学科学实验中心,甘肃兰州730050 [3]珠海市第二人民医院病理科,广东珠海519020

出　　处：《癌症》2009年第5期478-482,共5页Chinese Journal of Cancer

基　　金：全军"十一五"医学科学基金(06MA084)~~

摘　　要：背景与目的:高危型人乳头瘤病毒(human papillomavirus,HPV)以16型最为常见,HPV16 E6是宫颈癌主要的致癌基因之一,特定的E6突变是宫颈癌发生的主要因素之一。本研究主要观察兰州地区宫颈癌组织中是否存在E6突变,并探讨了突变与宫颈癌发生的关系。方法:以23例宫颈癌手术切除标本及5例正常宫颈组织的DNA作为模板,PCR扩增HPV-16 E6基因201-523位,PCR产物直接测序,分析其HPV16E6基因的突变规律。结果:PCR扩增结果表明5例正常宫颈组织中HPV16 E6阳性率为0%(0/5),宫颈癌组织中HPV16 E6阳性率为82.61%(19/23),对18例样本PCR产物的测序和序列分析结果表明,6例(33.33%)E6基因与原型相同,12例(66.67%)E6基因发生突变,其中11例(61.11%)发生了350G突变。同时,在1例样本(5.56%)发现了249G突变。结论:兰州地区宫颈癌组织中存在着非常高的HPV感染率,且多数HPV16 E6发生了突变。Background and Objective. Human papillomavirus （HPV） 16 is the most common type of high-risk human HPVs. HPV16 E6 gene and its specific mutations are considered as risk factors causing cervical carcinoma （CC）. This study was to investigate HPV16 E6 mutations in Lanzhou region and explore the relationship between HPV16 E6 mutations and the development of CC. Methods= Tissue DNA was extracted from 23 patients operated on for CC and five normal cervical controls. The partial sequence of the HPV16 E6 gene （nucleotide 201-523） was amplified by PCR from the tissue DNA extracted from the samples. PCR fragments were sequenced and analyzed. Results= The positive rates of HPV16 E6 in five normal cervical and 23 CC tissues were 0 （0/5） and 82.61% （19/23）, respectively. Prototype HPV16E6 gene was found in six cases （33.33%） while mutation in the E6 gene was detected in 12 cases （66.67%）, among which a 350G mutation was found in 11 cases （61.11%）. Moreover, a 249G mutation was identified in one CC case （5.56%）. Conclusions： There is a high HPV infection rate in CC tissues in Lanzhou region, and most of the HPV16E6 are mutated.

关 键 词：宫颈肿瘤 人乳头瘤病毒 E6基因 突变 

分 类 号：R737.33[医药卫生—肿瘤]