Span-20和Tween-80对发酵液中Nisin泡沫分离的影响  

Effects of Span-20 and Tween-80 on Foam Fractionation Behavior of Nisin in Fermentation Broth

作　　者：李新涛[1] 吴兆亮[1] 赵艳丽[1] 武亚明[1] 

机构地区：[1]河北工业大学化工学院生物工程系,天津300130

出　　处：《过程工程学报》2009年第2期279-283,共5页The Chinese Journal of Process Engineering

基　　金：天津市应用基础及前沿技术研究计划重点基金资助项目(编号:08JCZDJC25200);河北省教育厅自然科学研究计划资助项目(编号:Z2008310)

摘　　要：研究了微量非离子表面活性剂Tween-80和Span-20对发酵液中乳链菌肽(Nisin)泡沫分离的影响.结果表明,发酵液泡沫分离时泡沫层轴向气泡直径、连续通气过程的泡沫层高度、泡沫层平均持气量等均因添加活性剂而明显变化.不同体系气泡大小为,含0.2g/LSpan-20发酵液>含0.5g/LSpan-20发酵液>发酵液>含0.2g/LTween-80发酵液>含0.5g/LTween-80发酵液;Tween-80的加入破坏了气泡表面的网状结构,因而泡沫层持气量较高;连续通气时,2种表面活性剂都会引起泡沫层高度降低,但Tween-80会增加泡沫层的稳定性.当Tween-80浓度达0.5g/L时,Nisin的收率大幅度提高,原因是分离过程中Nisin失活率减少.The effects of Tween-80 and Span-20 on foam fractionation of Nisin （a food additive） fermentation broth were studied by using a single foam column. The results indicated that bubble size distribution, foam height and average gas holdup of the foam layer of the mixed system containing Tween-80 and Span-20 changed obviously from Nisin fermentation broth. The bubble size order of different mixed systems was： fermentation broth containing 0.2 g/L Span-20〉fermentation broth containing 0.5 g/L Span-20〉fermentation broth〉fermentation broth containing 0.2 g/L Tween-80〉fermentation broth containing 0.5 g/L Tween-80. The addition of surfactants decreased the height of foam layer and gas holdup, but Tween-80 increased the foam stability. And Tween-80 reduced the denaturation rate of Nisin in foam fractionation, so the separation effect was improved distinctly.

关 键 词：泡沫分离 非离子表面活性剂 乳链菌肽 

分 类 号：TQ028.8[化学工程]