紫外-亚硝基胍复合诱变选育海藻糖合酶高产菌株的研究  

Breeding of a high trehalose Synthase-producing strain by UV-NTG composite mutation

作　　者：井瑞洁[1] 马春玲[1] 丁振[2] 王腾飞[1] 王瑞明[1] 

机构地区：[1]山东轻工业学院食品与生物工程学院,济南250353 [2]日照职业技术学院食品工程学院,日照276826

出　　处：《食品科技》2009年第5期16-20,共5页Food Science and Technology

基　　金：山东省优秀中青年科学家基金项目(BS0384)

摘　　要：以海藻糖合酶产生菌株P06为出发菌株,经过紫外线与亚硝基胍复合诱变最终选育出一株海藻糖合酶高产突变株P06-3-5。与出发菌株相比,其产酶能力提高了1.25倍。突变株P06-3-5经10余代传代实验,具有良好的遗传稳定性。并采用红外吸收光谱对海藻糖成品进行了分析。Based on P06, the mutant strain P06-3-5 which was treated with ultraviolet and NTG inducement was screened and got. The ability of the mutant to produce trehalose synthase increased 1.25 times compared with P06. P06-3-5 had a good genetic stability after 10 generations by transfer of culture experiment. Meanwhile the finished product of trehalose was analysised with infrared absorption spectrum.

关 键 词：海藻糖 突变株P06—3—5 复合诱变 红外光谱 

分 类 号：TS201.3[轻工技术与工程—食品科学]