漏斗多孔菌液体发酵产漆酶条件研究  

Production of laccase from Polyporus arcularius A08 by submerged fermentation

作　　者：王剑锋[1] 王璋[2] 李江[1] 饶军[1] 

机构地区：[1]东华理工大学生物系,抚州344000 [2]中国食品发酵工业研究院,北京100027

出　　处：《菌物学报》2009年第3期440-444,共5页Mycosystema

摘　　要：采用单因子实验分析了培养基成分及初始pH对漏斗多孔菌液体发酵产漆酶的影响。结果表明,米糠和甘蔗半纤维素组成复合碳源、NH4Cl和高C/N有利于漆酶的产生,Cu2+、米糠水解液和萘乙酸对漆酶合成具有诱导作用,1-萘酚、愈创木酚、联苯胺、乙醇、吐温80等抑制漆酶的合成,Cu2+和萘乙酸同时存在时也限制漆酶的产生,产酶培养基最适初始pH为5.2-5.7。利用优化的产酶培养基液体摇瓶培养漏斗多孔菌A08,产酶活力提高0.95倍,达到1480U/L。By using single-factor analysis, the effects of the medium composition and initial pH on production of laccase from Polyporus arcularius A08 cultivated in liquid medium was investigated. The results showed that its productivity of laccase was improved when adding NH4Cl and mixed carbon source containing rice bran and hemicellulose in the medium with high C/N. The laccase production in Polyporus arcularius A08 was significantly induced by addition of some substance such as rice bran hydrolysate, Cu^2＋ or naphthylacetic acid （NAA） in the medium, but obviously inhibited byl-naphthol, benzidine, guaiacol, ethanol tween 80 and the combination of Cu^2＋ and NAA, respectively. In addition, the suitable initial pH for laccase production was 5.2--5.7 As the result, the laccase productivity of Polyporus arcularius A08 with submerged shaking culture with the utilization of the optimized fermentation medium was increased 0.95 times reaching an enzyme activity of 1480U/L.

关 键 词：生长调节剂 米糠水解液 诱导物质 半纤维素 

分 类 号：TQ925[轻工技术与工程—发酵工程]