灵芝生物转化蛋白核小球藻发酵粗多糖的抑瘤实验  

Anti-tumor effect of polysaccharides from ferment liquid of bioconverted Chlorella pyrenoidosa by Ganoderma lucidum

作　　者：杨从发[1,2] 丁重阳[1] 章克昌[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [2]淮海工学院海洋学院,江苏连云港222000

出　　处：《中国酿造》2009年第5期83-86,共4页China Brewing

摘　　要：在灵芝基础培养基中添加小球藻粉,灵芝能对小球藻细胞进行生物破壁,同时进行生物转化。藻粉添加量以10g/L为最佳,产生的多糖最高达0.29mg/L。提取转化的多糖,并与灵芝多糖、小球藻提取多糖的混合物进行抗肿瘤实验。结果表明,产生的转化多糖抗肿瘤效果最好,在最适剂量(0.2g/kg.d)时,抑瘤率为762.%。Appending Chlorella pyrenoidosa powder by 10g/L in the basic medium of Ganoderma lucidum could enhance the polysaccharides yield to 0.29mg/L by bioconversion. In order to study the anti-tumor activity of different polysaccharides （from fermentation liquid of bioconverted Chlorella pyrenoidosa by Ganoderma lucidum, from fermentation liquid of Ganoderma lucidum in basic madium, from Chlorella pyrenoidosa, or mixed polysaccharides） , an experiment was carried out in vivo. The results showed the bioconverted polysaccharides had the best anti-tumor activity. The anti-tumor inhibition was 76.2% at the optimum dose （0.2g/kg· d ）.

关 键 词：灵芝 蛋白核小球藻 多糖 抗肿瘤 

分 类 号：TQ929[轻工技术与工程—发酵工程]