发状念珠藻多糖和细胞培养液对葡萄糖浓度测定的影响  

Effects of nosoflan and Nostoc flagelliforme cell cultures on the determination of glucose concentration

作　　者：许鹏[1] 贾士儒[1] 谭之磊[1] 王静文[1] 丛茂林[1] 

机构地区：[1]天津科技大学教育部工业微生物重点实验室,天津300457

出　　处：《中国酿造》2009年第5期144-146,共3页China Brewing

基　　金：国家重点基础研究发展计划"973计划"(2007CB714305);国家自然科学基金(20776112)

摘　　要：采用生物传感仪测定法和DNS法,对加入发菜多糖和发菜细胞培养发酵液的葡萄糖溶液进行了浓度测定,研究了多糖和细胞培养液对葡萄糖浓度测定的影响作用。结果表明,发菜多糖对葡萄糖浓度测定无影响。添加发菜混合营养培养液后测得葡萄糖浓度为标准葡萄糖溶液与发酵液中葡萄糖浓度之和。The effects of nosoflan and Nostoc flagelliforme cell cultures on the determination of glucose concentration were investigated by applying biological sensor and 3,5-dinitrosalicylic acid method （DNS）. The results showed that adding nosoflan had no effect on the determination of reducing sugar concentration. After adding nostoc flagelliforme cell cultures into the standard glucose solution, the gross glucose concentration measured by the method of biological sensor and DNS was the sum of glucose in cell cultures and in standard solution.

关 键 词：发状念珠藻 发菜多糖 发酵液 葡萄糖浓度 

分 类 号：O657.32[理学—分析化学]