大豆卵磷脂的提纯与磷脂酰胆碱含量的分析  

Study on Purification and Analysis of Phosphatidylcholine in Soybean Lecithin

作　　者：吴丽芹[1] 邵荣[2] 云志[1] 

机构地区：[1]南京工业大学化学化工学院,江苏南京210009 [2]盐城工学院化学与生物工程学院,江苏盐城224051

出　　处：《化学世界》2009年第5期276-279,共4页Chemical World

摘　　要：全溶剂法从大豆浓缩磷脂中提取卵磷脂,采用丙酮脱油、乙醇溶解、三氧化二铝脱色的工序,着重考察了丙酮的脱油条件对丙酮不溶物和卵磷脂收率的影响,最佳脱油条件:水浴温度45°C为宜,脱油时间60 min,丙酮用量150 mL,脱油次数3次。采用高效液相色谱分析磷脂酰胆碱的含量,结果表明,采用甲醇/乙腈/水为流动相可以分析磷脂酰胆碱的含量,并且稳定性和重现性都比较好。The extraction of phosphatidyleholine（PC） from crude soybean lecithin with some solvents such as acetone and alcohols was studied experimentally. The results showed that the best conditions of removing oil were： 45 ℃, 60 min, 150 mL and extracting three times. In HPLC determination of lecithin, good separation was obtained by using methanol/acetonitrile/water as the mobile phase. It showed that the stability and reproducibility were relatively well.

关 键 词：卵磷脂 提纯 高效液相 分析 

分 类 号：TQ645.9[化学工程—精细化工]