高浓度小新月菱形藻保存方法的研究  

Studies on the methods for storing Nitzschia closterium f.minutissima

作　　者：孙建华[1] 王如才[1] 赵强[1] 

机构地区：[1]青岛海洋大学水产学院

出　　处：《海洋学报》1998年第2期108-112,共5页

基　　金：山东省科委资助项目

摘　　要：对小新月菱形藻（Ｎｉｔｚｃｈｉａｃｌｏｓｔｅｒｉｕｍｆ．ｍｉｎｕｔｉｓｓｉｍａ）的保存方法进行了研究，试验证明，小新月菱形藻用不同的方法保存３个月，其最高存活率分别为：（１）在２０℃下，１５．０％的ＳＡＭ８组为７６．４％，１．５％的抗生素组为５４．６％，０．１％的防腐剂组为４５．３％，对照组仅为３５．４％。（２）在０℃下，１０．０％的ＳＡＭ８组为９８．４％，１．５％的抗生素组为７８．８％，０．１％的防腐剂组为６３．１％，低于对照组（６５．５％）．（３）－３０℃下，１０．０％的ＤＭＳＯ组为８４．６％，１０．０％的甘油组为８３．１％，对照组为４６．０％。为微藻的浓缩保存提供了新的方法。This study deals with the storage of Nitzschia closterium f. minutissima. After three months' storage , the following results have been carried out : (1) When t= 20℃ , the viability rate of N. closterium f. minutissima was 76. 4% in 15% SAM8 group , 54. 6% in 1. 5% antibiotic group , 45.3% in 0. 1% antiseptic group , and 35. 4% in control group. (2) When t= 0℃ , the viability rate of N. closterium f. minutissima was 98. 4% in 10% SAM8 group, 78.8% in 1. 5% antibiotic group, 63.1% in 0.1% antiseptic group, and 65.5% in control group. (3) When t=-30℃ , the viability rate of N. closterium f. minutissima was 84. 6% in 10% DMSO group , 83.1% in 1 0% glycerine group , and 46.0% in control group .

关 键 词：小新月菱形藻 保存方法 藻 贮存 防腐剂 

分 类 号：S984.4[农业科学—捕捞与储运]