紫菜中有机氯农药和多环芳烃的气相色谱-质谱联用测定法  

Determination of Organic Chloride Pesticides and Polycyclic Aromatic Hydrocarbons in Prophyra by Gas Chromatograph-Mass Spectrometry

作　　者：王娟[1] 杜鹃[2] 李梦滢[2] 洪颖[2] 程汉宁[2] 倪延延[2] 范春[1] 

机构地区：[1]厦门大学医学院预防医学系,福建厦门361005 [2]厦门大学医学院2005级预防医学专业本科生,福建厦门361005

出　　处：《环境与健康杂志》2009年第5期448-450,共3页Journal of Environment and Health

基　　金：厦门大学新世纪优秀人才支持计划

摘　　要：目的建立测定紫菜中有机氯农药和多环芳烃的气相色谱-质谱分析法。方法紫菜样品经过丙酮-正己烷（体积比为7：3）提取、C18固相萃取柱净化后，采用气相色谱．色谱联用进行定性和定量。结果有机氯农药和多环芳烃的线性范围均为0．01～5．0ug/ml，方法的检出限分别为0．05～0．32ng/g（干重）和0．25～0．56ng／g（干重）；有机氯农药的回收率为74．27％-121．49％，平均回收率为91．95％，RSD为3．19％～17．31％；多环芳烃的回收率为65．10％-119．26％，平均回收率为95．60％，RSD为2．75％～14．11％。结论该方法线性关系良好，精密度较高，具有良好的回收率和重复性，可用于紫菜样品中有机氯农药和多环芳烃的检测。Objective To develop an analytical method by gas chromatograph-mass spectrometry （GC-MS） for the determination of organic chloride pesticides and polycyclic aromatic hydrocarbons in prophyra Methods The samples were extracted by an acetone-hexane （7：3, V/V） mixture first, then were purified by C18 solid-phase extraction （SPE）, and then were determined with GC-MS. Results The linear range was 0.01-5.0 ug/ml, the limits of detection were 0.05-0.32 ng/g （dry weight） and 0.25-0.56 ng/g （dry weight） for organic chloride pesticides and polycyclic aromatic hydrocarbons respectively. The recovery rates and relative standard deviation for organic chloride pesticides were 74.27%-121.49% and 3.19 %-17.31% relatively; the recovery rates and relative standard deviation for polycyclic aromatic hydrocarbons were 65.10%-119.26% and 2.75%-14.11% relatively. Organic chloride pesticides were partly detected, but the detection rate of polycyelic aromatic hydrocarbons was higher. Fifteen polycychc aromatic hydrocarbons were detected in all samples but dibenz （a,h）-anthracene. Conclusion This method is sensitive, accurate and applicable to the determination of organic chloride pesticides and polycyclic aromatic hydrocarbons in prophyra.

关 键 词：色谱法 气相 农药 紫菜 有机氯农药 多环芳烃 气相色谱-质谱联用 

分 类 号：O657.7[理学—分析化学]