表达磷脂酰丝氨酸合成酶基因工程菌发酵条件的研究  

Fermentation Conditions for Phosphatidylserine Synthase Production by a Recombinant Strain

作　　者：王建玲[1] 张业尼[1] 路福平[1] 李玉[1] 

机构地区：[1]天津市工业微生物重点实验室,天津科技大学生物工程学院,天津300457

出　　处：《现代食品科技》2009年第5期515-518,共4页Modern Food Science and Technology

基　　金：天津科技大学研究基金项目资助(项目编号:20080202)

摘　　要：研究表达磷脂酰丝氨酸合成酶基因工程菌的发酵条件。利用250mL三角瓶发酵,对携带磷脂酰丝氨酸合成酶基因重组质粒的工程菌进行诱导表达,研究培养基的pH值、装液量、转速、诱导时菌体浓度、诱导剂浓度、诱导时间和温度对磷脂酰丝氨酸合成酶酶活的影响。确定工程菌的最佳发酵条件为:培养基pH值为7.5,装液量为40mL,转速为200r/min,诱导时菌体浓度为A600≈0.4,诱导剂蔗糖的终浓度为60mmol/L,诱导时间为27h,诱导温度为37℃。在最佳发酵条件下,酶活可达2.56U/mL,是优化前的1.72倍,为中试放大提供了理论依据。The fermentation conditions by recombinant Bacillus subtilis expressing Phosphatidylserine synthase （PSS） were studied. The influence of medium pH, volume of medium, rotation speed, cell concentration, revulsivum concentration, induction time and temperature on the activity of the PSS were analyzed and optimized in 250 mL shake flask scale. The best fermentation conditions were as follows： pH 7.5, volume of medium 40 mL, rotation speed 200 r/min, cell concentration A600≈0.4, sucrose 60 mmol/L, inducing temperature 37℃ and inducing time 27 h. Under the optimal conditions, the PSS activity was 2.56 U/mL, 1.72 times higher than that in the initial culture conditions. This research supplied references for pilot-plant-scale fermentation by recombinant bacteria.

关 键 词：磷脂酰丝氨酸合成酶 基因工程菌 发酵 条件优化 

分 类 号：Q78[生物学—分子生物学]