枯草杆菌JAS株α—淀粉酶的产生与其抗衣霉素的关系  

THE RELATION BETWEEN BACILLUS SUBTILIS JAS α-AMYLASE PRODUCTION AND ITS TUNICAMYCIN RESISTANCE

作　　者：周和山[1] 涂祖新[1] 

机构地区：[1]江西省科学院微生物研究所

出　　处：《江西科学》1990年第4期55-58,共4页Jiangxi Science

摘　　要：为了研究Bacillus subtilis的产α-淀粉酶性状与其抗衣霉素性状之间的关系,进一步选择在诱变育种时能有效地筛选淀粉酶基因突变株的方法,我们对此作了初步探索.衣霉素敏感型的α-淀粉酶工业生产菌B.subtilis JAS经过紫外线诱变后,选得9株抗衣霉素突变株.通过对它们进行的多次发酵酶活力测定,发现其中3株是α-淀粉酶缺陷型、3株酶活力基本不变或降低,另外3株酶活力明显提高,其中1株酶活力是出发菌株的114%—123%.试验表明B.subtilis α-淀粉酶的产生与其抗衣霉素基因的表达有着密切关系.因此,在对该菌的诱变育种时,可以采用衣霉素为粗筛选择压力.In order to understand the relationship between α-amylase production and Tunicamycin resistance of Bacillus subtilis and to chose an efficient method to select the B, subtilis α-amylase gene mutants after being treated by mutagen,this work was carried out. Industrial production strain B. subtilis JAS sensitive to Tunicamycin was treated by ultraviolet and the 9 mutants were selected on the Tunicamycin plate. After determination of their α-amylase activity, three of them were deficiency of α-amylase, three of them had similar or lower α-amylase activity, other three had higher α-amylase activity including one with 114-124% activity compared with their parental strain. The research showed that the α-amylase production by B, subtilis was highly related with the expression of Tunicamycin resistant gene. Therefore, it is quite possible to select mutants of α-amylase gene by using Tunicamycin.

关 键 词：Α-淀粉酶 衣霉素 枯草杆菌 

分 类 号：TQ925.1[轻工技术与工程—发酵工程]