人大肠癌组织中磷酸化哺乳动物雷帕霉素靶蛋白的表达及意义  

Expression of phosphorylated mammalian target of rapamycin in colorectal cancer and its significance

作　　者：项洪刚[1] 胡志前[1] 王伟军[1] 徐健[1] 张剑[1] 许超[1] 王强[1] 

机构地区：[1]第二军医大学长征医院普通外科,上海200003

出　　处：《第二军医大学学报》2009年第5期517-520,共4页Academic Journal of Second Military Medical University

摘　　要：目的:探讨哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)及其活化形式磷酸化mTOR(phos-phorylated mTOR,p-mTOR)在人大肠癌发生发展中的作用及临床意义。方法:应用组织芯片和免疫组织化学技术检测185例大肠癌组织及癌旁组织中mTOR及p-mTOR的表达,并分析其与大肠癌临床病理学特征如年龄、性别、肿瘤浸润深度(T分期)、淋巴转移、TNM分期和分化程度之间的关系。结果:在大肠癌旁组织中mTOR呈现弥散表达,p-mTOR基本不表达或散在表达,两者在大肠癌组织中的表达强度明显高于癌旁组织。mTOR和p-mTOR在大肠癌中的过表达率分别为45.9%和42.2%。两者均与性别、年龄无显著相关。mTOR的过表达与大肠癌的分化程度相关(P<0.05),而与浸润深度(T分期)、TNM分期、淋巴转移无显著相关(P>0.05)。p-mTOR的过表达与大肠癌的浸润深度(T分期)、淋巴转移和TNM分期显著相关(P<0.05),而与分化程度无显著相关(P>0.05)。结论:p-mTOR的过表达与人大肠癌的恶性表型密切相关,mTOR的磷酸化可能参与调控了大肠癌的发生发展。Objective： To explore the roles of mammalian target of rapamycin （mTOR） and the activated mTOR （phosphorylated mTOR, p-roTOR） in the development and progression of coloreetal cancer, and to discuss the clinical significance. Methods：The expression of mTOR and p-roTOR in 185 colorectal cancer specimens and the corresponding adjacent tissues were evaluated by tissue microarray and immunohistoehemistry,and the relationship between the expression and the age, sex, invasion depth（T stage）, lymph metastasis, TNM stage and differentiation degree was analyzed. Results： Diffused expression of mTOR and hardly any expression of p-roTOR were found in the adjacent tissues. The expression of roTOR and p-roTOR was obviously stronger in the colorectal cancer tissues compared with that in the adjacent tissues. The over-expression rates of roTOR and p-roTOR in colorectal cancer were 45.9% and 42.2%, respectively. There was no significant correlation of mTOR and p-roTOR over-expression with age,sex（P〈0.05）; the over-expression of roTOR was correlated with the differentiation degree （P〈0.05）, but not with the invasion depth （T stage）,TNM stage,or lymph metastasis （P〉0. 05）. The correlation of p-roTOR over-expression with the invasion depth （T stage）,lymph metastasis and TNM stage was significant （P〈0. 05） ,but that with the differentiation degree was not significant （P〉0.05）. Conclusion： Over-expression of p-roTOR is closely associated with the malignant phenotype of coloreetal cancer. It is also indicated that p-roTOR may be involved in the development and progression of colorectal cancer.

关 键 词：组织芯片 雷帕霉素 MTOR P-MTOR 结直肠肿瘤 

分 类 号：R735.34[医药卫生—肿瘤]